Pig protoheme extraction method

An extraction method, heme technology, applied in the direction of organic chemistry, etc., can solve the problems of co-production of other products, long production cycle, high cost, etc., and achieve the effect of omitting the steps of cracking and dissolving, saving production cost and saving time

Inactive Publication Date: 2016-06-15
FUJIAN HUAGENE BIOSCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] 1. The existing method has high cost and single product, and fails to co-produce other products;
[0007] 2. The use of highly toxic and difficult-to-recycle organic solvents in the extraction process cannot achieve environmentally friendly production;
[0008] 3. Low product purity, high extraction cost, long production cycle, difficult solvent recovery, etc.
The above method uses fresh pig blood as a raw material, and undergoes enzymatic hydrolysis and purification to obtain hemoglobin. The workload of filtration and centrifugation in the middle is quite large, which makes the production cycle of hemoglobin longer

Method used

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  • Pig protoheme extraction method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Step 1, take by weighing the production SOD waste residue of 100kg;

[0053] Step 2, add 200L of acidic acetone, homogenate, the volume ratio of concentrated hydrochloric acid and acetone in the acidic acetone is 2: 100, the concentration of hydrochloric acid in the described concentrated hydrochloric acid is 12mol / L;

[0054] Step 3, collecting the filtrate by plate and frame filtration;

[0055] Step 4, the filter residue repeats steps 2-3 once, merges the filtrate twice, and collects the filter residue for producing peptone;

[0056] Step 5, adjust pH to neutrality with sodium acetate;

[0057] Step 6, centrifuge and collect about 16 kg of sediment in a three-legged centrifuge;

[0058] Step 7, dissolve with 80kg0.1mol / L NaOH solution;

[0059] Step 8, centrifuging in a tubular centrifuge to discard the precipitate;

[0060] Step 9, adjust the pH to 4.0 with 0.1mol / L hydrochloric acid;

[0061] Step 10, centrifuging to collect the precipitate;

[0062] Step 11,...

Embodiment 2

[0065] Step 1, take by weighing the production SOD waste residue of 300kg;

[0066] Step 2, add 600L of acidic acetone, homogenate, the volume ratio of concentrated hydrochloric acid and acetone in the acidic acetone is 2: 100, the concentration of hydrochloric acid in the described concentrated hydrochloric acid is 12mol / L;

[0067] Step 3, collecting the filtrate by plate and frame filtration;

[0068] Step 4, the filter residue repeats steps 2-3 once, merges the filtrate twice, and collects the filter residue for producing peptone;

[0069] Step 5, adjust pH to neutrality with sodium acetate;

[0070] Step 6, centrifuge and collect about 46.5kg of sediment in a three-legged centrifuge;

[0071] Step 7, dissolve with 80kg0.1mol / L NaOH solution;

[0072] Step 8, centrifuging in a tubular centrifuge to discard the precipitate;

[0073] Step 9, adjust the pH to 4.0 with 0.1mol / L hydrochloric acid;

[0074] Step 10, centrifuging to collect the precipitate;

[0075] Step 11...

Embodiment 3

[0078] Step 1, take by weighing the production SOD waste residue of 500kg;

[0079] Step 2, add 1T of acidic acetone, homogenate, the volume ratio of concentrated hydrochloric acid to acetone in the acidic acetone is 2: 100, and the concentration of hydrochloric acid in the concentrated hydrochloric acid is 12mol / L;

[0080] Step 3, collecting the filtrate by plate and frame filtration;

[0081] Step 4, the filter residue repeats steps 2-3 once, merges the filtrate twice, and collects the filter residue for producing peptone;

[0082] Step 5, adjust pH to neutrality with sodium acetate;

[0083] Step 6, about 70kg of sediment is collected by centrifugation in a three-legged centrifuge;

[0084] Step 7, dissolve with 350kg0.1mol / L NaOH solution;

[0085] Step 8, centrifuging in a tubular centrifuge to discard the precipitate;

[0086] Step 9, adjust the pH value to 4.0 with 0.1mol / L hydrochloric acid;

[0087] Step 10, centrifuging to collect the precipitate;

[0088] Ste...

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Abstract

The invention provides a pig protoheme extraction method by which production cycle is short and protoheme purity is high. The pig protoheme extraction method includes the following steps: adding acid acetone into SOD (superoxide dismutase) production waste residues according to the weight ratio of 2:1, performing filtration after stirring, and collecting filter liquor; adding sodium acetate liquor into the filter liquor until the filter liquor becomes neutral, and collecting sediments after centrifugation; adding 0.05-0.15mol / L of NaOH liquor into the sediments according to the volume ratio of (4-6) :1 to dissolve the sediments, collecting the sediments after centrifugation, adding hydrochloric acid into the sediments until the pH value of the liquor becomes 4.0, collecting the sediments after centrifugation, and drying the sediments to obtain the finished protoheme. The pig protoheme extraction method has the advantages that the SOD production waste residues are subjected to purification by sodium acetate, sodium hydroxide and hydrochloric acid in sequence after being extracted by the acid acetone, the extraction time is short, the protoheme sediments are generated completely, and the purity of the obtained protoheme is improved greatly.

Description

technical field [0001] The invention relates to the technical field of heme preparation, in particular to a method for extracting porcine heme. Background technique [0002] Hemoglobin in blood is composed of 4 molecules of heme and 1 molecule of globin. Blood is an animal protein resource with high nutritional value, which can be widely used in the food industry and has a very rich source. Heme is an important class of natural porphyrin-iron compounds. It is the prosthetic group (active site) of hemoglobin, myoglobin and red blood cells. It is a natural pigment in animal blood and has important physiological functions and use values. [0003] The hemoglobin in pig blood is not easily digested and absorbed, has a bloody smell, poor palatability and has a darker color, so for a long time, it has been less used in food and more discarded, which not only pollutes the environment, but also wastes precious protein resources. In today's shortage of animal protein resources, the...

Claims

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Application Information

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IPC IPC(8): C07D487/22
CPCC07D487/22
Inventor 黄发灿郑登忠王光煌章丽丽
Owner FUJIAN HUAGENE BIOSCI CO LTD
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