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Traditional Chinese medicine extract with anti-depression effect and application thereof

An extract and anti-depressant technology, applied in the field of traditional Chinese medicine extract with anti-depressant effect and its preparation, can solve the problems affecting the treatment and rehabilitation of patients with depression, achieve significant anti-depressant activity, convenient clinical use, and increase expression Effect

Inactive Publication Date: 2016-06-15
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective drug for this target, which seriously affects the treatment and rehabilitation of patients with depression.

Method used

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  • Traditional Chinese medicine extract with anti-depression effect and application thereof
  • Traditional Chinese medicine extract with anti-depression effect and application thereof
  • Traditional Chinese medicine extract with anti-depression effect and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A kind of Chinese medicine extract with antidepressant effect, it is characterized in that, this Chinese medicine extract is made by following method:

[0030] Step 1: Select the following raw materials: 300 parts of ginseng, 200 parts of Polygala, 200 parts of Shichangpu, and 300 parts of Poria cocos;

[0031] Step 2: Take the raw material drug in step 1, first add extraction solvent water with volume 8 times the weight of the raw material drug of traditional Chinese medicine, reflux extraction at 100°C for 120min, filter and add extraction solvent water with volume volume 10 times the weight of medicinal material, and reflux extraction at 100°C for 120min . Concentrate the extract to obtain a concentrated solution with a concentration of 1.0 g / mL;

[0032] Step 3: Take the concentrated solution of step 2 and elute it through a macroporous resin column. The elution conditions of the macroporous resin are: select 1.25L860021 macroporous adsorption resin, the adsorption...

Embodiment 2

[0033] Example 2 Antidepressant Experimental Research of Chinese Medicine Extract

[0034] 1. Experimental materials and drugs

[0035] 1. Experimental equipment

[0036] Animal multiple behavior tester (Shanghai Jiliang Instrument Co., Ltd.); BT125 electronic balance (Sartorius Scientific Instrument Co., Ltd.); KQ-250E ultrasonic cleaner (Kunshan Hechuang Ultrasonic Instrument Co., Ltd.).

[0037] 2. Drugs

[0038] Fluoxetine was purchased from TCI (Shanghai) Chemical Industry Development Co., Ltd.

[0039] Test drug: the Chinese medicine extract prepared in Example 1 of the present invention (low dose, medium dose, and high dose are 1, 3, and 10 ug / mL, respectively).

[0040] 3. Experimental animals

[0041] SPF grade male ICR mice, weighing 18-22 g, were purchased from the Experimental Animal Center of Nanjing University of Traditional Chinese Medicine, certificate number: SCXK (Su) 2015-0001.

[0042] 2. Experimental method

[0043] 1. Mouse tail suspension test

[00...

Embodiment 3

[0054] Example 3 Experimental Study on the Effect of Chinese Medicine Extracts on Neurotransmitters in the Brain of Depressed Animals

[0055] 1. Experimental materials and drugs

[0056] 1. Experimental equipment

[0057] Agilent1290Series high performance liquid chromatography; AgilentQQQ6410A triple quadrupole mass spectrometer; analytical column: ACEC18-AR, 1.8μm, 2.1x100mm; BT125 electronic balance (Sartorius Scientific Instrument Co., Ltd.); ultrapure water preparation instrument (Nanjing Yipu Yida Corporation).

[0058] 2. medicine: standard substance dopamine, norepinephrine, 5-hydroxytryptamine are all purchased from Sigma-Aldrich company; Tested medicine: the Chinese medicine extract that the embodiment of the present invention 1 prepares (low dose, medium dose, high dose are respectively 1, 3, 10ug / mL).

[0059] 2. Experimental method

[0060] 1. Sample Processing

[0061] The mice that completed the behavioral test were killed and dissected to take the whole b...

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Abstract

The invention discloses a traditional Chinese medicine extract with an anti-depression effect and application thereof. 300 to 600 parts of ginseng root, 200 to 400 parts of thinleaf milkwort root, 200 to 400 parts of grassleaf sweelflag rhizome and 300 to 600 parts of Indian bread as active pharmaceutical ingredients are added with water and extracted, and extract is taken out and concentrated, so that concentrate is obtained; the concentrate is purified by a macroporous resin column, water is first used for removing impurities, ethanol of optimal volume is then adopted for elution, so that eluent is obtained, and the traditional Chinese medicine extract is obtained by concentration. Demonstrated by a mouse tail suspension and swimming depression model and PC12 cell and C6 cell models, the traditional Chinese medicine extract provided by the invention has an effect of obviously improving depressive emotion, inhibiting neurotransmitter degradation and promoting neurotrophic factor expression, the therapeutic effect is reliable, safety is high, adverse reaction is low, and the traditional Chinese medicine extract can be used for preparing anti-depression drugs.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a traditional Chinese medicine extract with antidepressant effect and its preparation method and application. Background technique [0002] Depression is a disease characterized by significant and persistent low mood, accompanied by insomnia, memory loss, eating disorders and other symptoms. In severe cases, suicidal thoughts and behaviors may appear. It has been reported that 16% of the population will experience depressive symptoms at some point in their lives. If these symptoms persist for more than two weeks without significant improvement, the patient can be diagnosed with depression. According to the statistics of the World Health Organization, depression has become the fourth largest disease in the world, and it is estimated that by 2020, it may become the second largest disease after coronary heart disease. Depressed patients cannot adapt to society and have dec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/888A61P25/24
CPCA61K36/888A61K36/076A61K36/258A61K36/69A61K2236/331A61K2236/55A61K2300/00
Inventor 朱悦詹华强
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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