Preparation method of gynura nepalensis total flavones
The technology of total flavonoids of Panax notoginseng and chrysanthemum of Nepal, applied in the field of bioengineering, can solve the problem of low purity of total flavonoids of chrysanthemum of Panax notoginseng, and achieve the effects of remarkable technological progress, mild conditions and simple steps.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] The determination of total flavonoids is represented by the amount of rutin contained in the sample, and rutin is used as a standard product to make a standard curve: use 80% ethanol as a solvent, and accurately prepare a 0.2mg / ml rutin standard solution for use, and take the above standard solution 0.0, 1.0, 2.0, 4.0, 8.0, 10.0ml are placed in a 25ml volumetric flask, add 80% ethanol to prepare 10ml, first add 1ml of 5% sodium nitrite solution, mix well, let stand for 6min; add 1ml of 10% aluminum nitrate solution, shake well and Stand for 6min; add 10ml of 4% sodium hydroxide solution. Finally, place the scale with 80% ethanol at a constant volume for 15 minutes to measure the absorbance at 510 nm and draw a standard curve:
[0023] Y=0.5205X+0.0237,R 2 =0.9999 (X is the concentration of rutin mg / ml, Y is the absorbance value).
[0024] When measuring the sample, take 10ml sample and carry out according to the rutin assay method.
Embodiment 2
[0026] 1) Weigh 15 g of the dried leaves of Panax notoginseng from Nepal, and pulverize it into a powder with a particle size of 40 mesh;
[0027] 2) Add 450ml of ethanol with a mass percentage concentration of 95% to the Nepal chrysanthemum notoginseng obtained in step (1), stir evenly, and extract with ultrasonic waves for 45min at 60°C, filter after extraction, collect the upper filtrate, and refill it in the filter residue Add 300ml of ethanol with a mass percentage concentration of 95%, stir evenly, and then extract with ultrasonic wave at 60°C for 45min, filter after extraction, collect the filtrate, and combine the filtrate collected twice;
[0028] 3) Concentrate the combined filtrate obtained in step (2) to 20ml under reduced pressure, control the temperature during the concentration process to be 50°C, and the pressure to be -0.08~-0.1Mpa; determine the total flavonoids of the concentrated solution (see Table 2-1 for experimental data ), the extraction rate of flavon...
Embodiment 3
[0033] 1) Weigh 15 g of the dried leaves of Panax notoginseng from Nepal, and pulverize it into a powder with a particle size of 60 mesh;
[0034] 2) Add 300ml of ethanol with a mass percentage concentration of 80% to the Nepalese chrysanthemum notoginseng obtained in step (1), stir well, and extract with ultrasonic waves for 45min at 60°C, filter after extraction, collect the filtrate, and add to the filter residue 200ml of ethanol with a mass percentage concentration of 80%, stir evenly, and then extract with ultrasonic wave at 60°C for 45min, filter after extraction, collect the filtrate, and combine the filtrate collected for the second time;
[0035] 3) Concentrate the combined filtrate obtained in step (2) to 20ml under reduced pressure. During the concentration process, the temperature is controlled at 50°C and the pressure is -0.08~-0.1Mpa; the samples are measured, and the results are shown in Table 2-2. The extraction rate calculation formula shows that the extractio...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com