A method of reducing the accumulation of cadmium in honeysuckle and increasing its yield by using Glomus intrarhizoi
A technology of Glomus intraroote and Glomus intraroote, which is applied in the fields of soil preparation methods, botanical equipment and methods, applications, etc., can solve the problems of reducing cadmium accumulation in honeysuckle, improve mineral nutrition status, and reduce operating costs , no secondary pollution effect
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[0045] 1. Propagation and culture of test strains and preparation of bacterial agents
[0046] Rhizophagus intraradices (Ri) BGC AH01 was provided by the Institute of Plant Nutrition and Resources, Beijing Academy of Agriculture and Forestry Sciences.
[0047] After using corn (Zea mays) as a host plant for potted propagation, the mixture containing host plant root segments, fungal hyphae, mycorrhizal fungal spores and potting soil is used as an inoculant. The specific process is as follows:
[0048] Fill the sterilized sand (according to the weight ratio, sand:soil=1:1) culture medium to 4 / 5 of the plastic basin. According to the inoculum amount of 4% (w:w), the inoculant of Glomus intraradices was evenly spread on the substrate as a thin layer. Then cover the sterilized substrate 2cm, water, sow 10-15 corn seeds / pot. Cover with 0.5cm sterilized substrate, and then move to the greenhouse for cultivation.
[0049] Harvest after 5 months of potting. When harvesting, first ...
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