Cancer detection kit based on large-scale data mining and detection method

A large-scale data and cancer detection technology, applied in the direction of electrical digital data processing, special data processing applications, instruments, etc., can solve the problems of insufficient sensitivity, ineffective detection of cancer, misjudgment, etc.

Inactive Publication Date: 2016-06-08
江苏格致生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biochemical markers include CEA, AFP, PSA, etc. The main defects of these markers are: (1) the types of cancer covered are limited, and most cancers cannot be effectively detected; (2) the specificity is poor, and some basic diseases, such as hepatitis, will Causes AFP to rise and cause misjudgment, prostatitis can also cause PSA to rise and cause misjudgment, etc.; (3) The half-life in the blood is too long, such as AFP protein can survive in the blood for 7 to 28 days or longer, it is difficult to For specific real-time tracking of surgical treatment, radiotherapy, drug treatment effect
Imaging techniques include B-ultrasound, CT and PET, as long as

Method used

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  • Cancer detection kit based on large-scale data mining and detection method
  • Cancer detection kit based on large-scale data mining and detection method
  • Cancer detection kit based on large-scale data mining and detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1: Peripheral blood collection, extracting free DNA with DNA extraction reagents

[0069] 1. Collect 4mL of peripheral blood from the subjects into EDTA anticoagulant tubes. The subjects were all voluntary, and the cancer samples came from the Affiliated Hospital of Jiangsu University.

[0070] 2. Within 4 hours of fresh blood, centrifuge at 1600g for 10 minutes, take the supernatant and transfer it to a 1.5mLEP tube, and the tip of the pipette should avoid touching the middle layer and the bottom layer of red blood cells.

[0071] 3. Centrifuge at 16000g for 10 minutes, transfer the supernatant to a new 1.5mLEP tube, and store in a -80°C refrigerator.

[0072] 4. Cell-free DNA extraction: standard Qiagen cell-free DNA extraction kit (QIAGEN, QiaAmpDNABloodMiniKit, 55114) was used to extract 1-50 ng DNA per 4 mL of peripheral blood according to the instructions.

[0073] The specific operation steps are as follows:

[0074] (1) After thawing a tube of plasma ...

Embodiment 2

[0087] Example 2: Using high-throughput sequencing library preparation reagents to perform DNA polymerase amplification and establish a sequencing library

[0088] The standard KAPA next-generation sequencing library kit (KAPA HyperPlus library preparation kit, 28100) was used to build a library on the obtained free DNA products and sequenced on a machine (using a high-throughput sequencer from Illumina).

[0089] In this embodiment, the specific operation steps are as follows

[0090] (1) End repair reaction:

[0091] Prepare the following reaction mixture:

[0092]

[0093] The reaction mixture was incubated at 20°C for 30 min; immediately purified (using Agencourt AMPure XP Beads kit, Cat. No. A63882) to obtain the product of the end repair reaction;

[0094] Make sure that AMPureXPBeads are equilibrated to room temperature; add AMPureXPBeads to the end repair and reaction, prepare the following reaction mixture:

[0095] End repair reaction product 100μL

[0096] AM...

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Abstract

The invention discloses a cancer detection kit based on large-scale mining and a detection method, and belongs to the technical field of biomedical detection. The kit comprises a DNA extraction reagent, a high-throughput sequencing library preparation reagent, gene sequence alignment software and chromosome cover degree calculation software. The method comprises the steps that firstly, peripheral blood is collected from a subject and plasma is separated out; DNA polymerase is used for amplification and a sequencing library is prepared; large-scale sequencing is performed on the prepared library; sequence alignment is performed on a sequencing result, statistic is performed on the distribution situation on a genome, and whether a cancer cell genomic sequence from tumor tissue exists or not is judged, so that whether a detection object carries a cancer or not is judged. According to the cancer detection kit and the detection method, the defect that existing cancer screening specificity and sensitivity are poor is overcome, the method causes no irradiation or wounds, and cancer detection can be achieved only through 4-10 mL of peripheral blood.

Description

technical field [0001] The invention relates to a cancer detection kit and detection method based on large-scale data mining, in particular to a high-throughput sequencing data mining and non-invasive cancer detection kit and detection method, belonging to biomedical detection technology. technical background [0002] Malignant tumors are often associated with changes in the number and structure of chromosomes, because there are generally two types of chromosomes in cells, some chromosomes carry genes that express malignant tumors, and other chromosomes carry genes that inhibit malignant tumors. If the chromosome is mutated, it will lead to an imbalance of genes on the chromosome, and a tumor will appear. [0003] Current cancer detection relies heavily on blood biomarkers and imaging techniques. Biochemical markers include CEA, AFP, PSA, etc. The main defects of these markers are: (1) the types of cancer covered are limited, and most cancers cannot be effectively detected;...

Claims

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Application Information

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IPC IPC(8): G06F19/22G06F19/24
CPCG16B40/00G16B30/00
Inventor 钱自亮郑媛虹魏国鹏
Owner 江苏格致生命科技有限公司
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