A lectin chip and method for detecting sugar chain markers based on salivary protein

A salivary protein and lectin technology, applied in the field of lectin chips based on protein detection of sugar chain markers, can solve the problem of damaging the binding stability of catenin and N-sugar chains, increasing the ability of cell transfer and invasion, and affecting the network of catenin Amino acid phosphorylation and other issues

Active Publication Date: 2018-05-04
深圳格道糖生物技术有限公司
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Problems solved by technology

[0006] For example, some functional sugar chains have been found in the research of gastric cancer, and the representative one is the up-regulated expression of N-acetylglucosaminyltransferase V (GnT-V) in gastric cancer cells, which leads to the synthesis of β1,6-linked N-acetyl Glucosamine branched N-glycan (β1,6GlcNAc branched N-Glycan), this branched sugar chain affects the phosphorylation of tyrosine in catenin, resulting in the damage of β-catenin and P120 protein, destroying catenin The stability of the combination with N-sugar chains affects the adhesion ability between cells and increases the ability of cell transfer and invasion

Method used

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  • A lectin chip and method for detecting sugar chain markers based on salivary protein
  • A lectin chip and method for detecting sugar chain markers based on salivary protein
  • A lectin chip and method for detecting sugar chain markers based on salivary protein

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Embodiment Construction

[0038] The present invention will be further described by the specific examples given below, but not as a limitation of the present invention.

[0039]A total of 12 healthy volunteers were selected and did not take any drugs within a week. There were 64 diagnosed gastric cancer patients (21 cases of gastric cancer stage I, 18 cases of stage II, 20 cases of stage III, and 5 cases of stage IV). The age distribution is as follows: Table 1 shows.

[0040] Table 1

[0041]

[0042] Healthy volunteers were used as the basic control group, and confirmed gastric cancer patients were used as the sample group.

[0043] (1) Saliva collection:

[0044] The above-mentioned people gargle with normal saline three times between 9 and 10 o'clock after meals, and then quickly collect the whole saliva secreted naturally. Collect at least 1ml of saliva and put it on ice immediately, and add protease inhibitors to prevent protein degradation. , add 1 μL per milliliter of saliva;

[0045] (2...

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Abstract

A lectin chip for detecting sugar chain markers based on salivary protein and a method thereof, relating to a lectin chip for detecting sugar chain markers, in particular to a lectin chip for detecting sugar chain markers based on protein and its method. The purpose is to provide a non-damaging lectin chip and method for detecting sugar chain markers by identifying proteins in saliva. The lectin chip for detecting sugar chain markers based on salivary protein includes a test lectin probe set, and the test lectin probe set includes faba bean lectin, Reticula aurantia lectin, pea lectin, square bean Lectin-II and lentil bean lectin-I, wherein lentil lectin-II and lentil bean lectin-I were used as internal reference lectins. Using the lectin chip to detect sugar chain markers has the advantages of no damage to the human body, less sample consumption, fast detection results and high sensitivity, and can be applied to identify whether there is gastric cancer.

Description

technical field [0001] The invention relates to a lectin chip for detecting sugar chain markers, in particular to a protein-based lectin chip for detecting sugar chain markers and a method thereof. Background technique [0002] Gastric cancer is a common malignant tumor with high morbidity and mortality worldwide, especially in East Asia, Eastern Europe, and South America due to the lack of significant early features. Currently commonly used diagnostic markers for gastric cancer include: CEA, CA19-9, CA72-4, CA125, AFP, etc., but all have limitations. Histopathological examination is still the gold standard for the diagnosis of gastric cancer at present. However, in view of the inherent defects of histological examination, such as destructive examination, dynamic detection, and sampling differences, it is important to seek non-invasive examination for early diagnosis of gastric cancer. The key to gastric cancer. [0003] With the progress of society and the development of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574
CPCG01N33/57446G01N2800/06
Inventor 李铮舒健于汉杰刘夏薇杜昊骐
Owner 深圳格道糖生物技术有限公司
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