Method for quickly classifying bacterial colonies on culture medium on basis of hyperspectral imaging technology

Abstract

The invention discloses a method for quickly classifying bacterial colonies on a culture medium on the basis of a hyperspectral imaging technology. The method comprises the steps that a hyperspectral imaging system is utilized to collect a reflecting image of the bacterial colonies on the culture medium, wherein the image comprises spectral information and image information of the bacterial colonies; the hyperspectral reflecting image is corrected through a black-white file, and a corrected image is obtained; the corrected image is processed through an image processing technology, and a mask image of the original hyperspectral image is obtained; spectral data information of each bacterial colony is extracted according to the positions where the bacterial colonies in the mask image are located; a full-wavelength linear prediction model based on bacterium categories and the spectral data information is built, and category prediction on an unknown bacterium sample is achieved through the model. In addition, multiple wavelength selection methods are utilized to optimize characteristic wavelengths, a corresponding simplified model is built, and the simplified model can also predict the category of the unknown bacterium sample. By means of the method for quickly classifying the bacterial colonies on the culture medium on the basis of the hyperspectral imaging technology, high-precision, quick and lossless identifying detecting and classifying of the bacterial colonies on the culture medium are achieved.

Description

technical field [0001] The invention relates to a method for rapidly classifying bacterial colonies on a culture medium, in particular to a method for rapidly classifying Escherichia coli, Listeria and Staphylococcus aureus colonies on a culture medium based on hyperspectral imaging technology. Background technique [0002] The ubiquity of bacteria in the environment determines the important role they play in human life. Among them, beneficial bacteria can promote human health, while pathogenic bacteria can cause adverse effects such as food-borne diseases. In particular, pathogenic bacteria will not only threaten human health, but also lead to waste of social resources. If pathogenic bacteria are used by terrorists, it will also induce social unrest. Therefore, it plays an extremely important role in the detection and identification of bacteria. [0003] With the development of science, bacterial detection methods based on polymerase chain reaction, enzyme-linked immunos...

AI Technical Summary

Problems solved by technology

However, the application of hyperspectral imaging technology to the classification and detection of bacterial colonies is rarely reported in China

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