Fluorescent quantitative PCR detection method for detecting colonization amount of tobacco anthracnose
A fluorescence quantitative and detection method technology, applied in the field of molecular biology, can solve the problems of indistinguishability, large error, time-consuming, etc., and achieve the effects of short detection time, reduced difficulty, and simple process
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[0040] 1. Extraction of DNA from susceptible tobacco strains
[0041] 1. Use scissors to cut the leaf tissue of the tobacco plant to an angular shape below 3mm, with a weight within the range of 10-100mg, put it into a 1.5ml Microtube, and freeze it at -20°C.
[0042] 2. Take out the frozen plant tissue and let it thaw at room temperature for about 5 minutes.
[0043]3. Gently centrifuge to collect the plant tissue at the bottom of the Microtube.
[0044] 4. Use the tip of the PipetTip to press the plant tissue to the bottom of the Microtube about 10 times for physical breaking.
[0045] 5. Add 400 μl of ExtractionSolution1 and shake vigorously for 5 seconds. If the plant tissue is still stuck at the bottom of the Microtube, flick the Microtube with your fingers to suspend it.
[0046] 6. Slightly centrifuge, add 80 μl of ExtractionSolution2, shake vigorously for 5 seconds. After adding ExtractionSolution2, a white precipitate was produced, and the solution became cloudy a...
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