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Method of preparing nano level bacterial cellulose from waste food

A technology of bacterial cellulose and waste, applied in the field of comprehensive utilization of food waste, can solve the problem of high cost, achieve the effect of increasing production and reducing carbon source cost

Inactive Publication Date: 2016-06-08
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the biggest problem encountered in the production of bacterial cellulose is the high cost. At present, carbon sources account for a large proportion of the production cost. Choosing a cheap raw material to replace the currently used starch can not only reduce the production cost of bacterial cellulose , and has a certain effect on reducing environmental pollution
It has not been reported that jujube juice waste, which is a cheap agricultural waste, is used as a raw material to comprehensively utilize and produce these biodegradable green products.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Extraction of jujube juice

[0021] Put 100g of red dates in 1000L of water, boil at 100°C for 20 minutes, let it cool down, and filter through a sieve to obtain jujube juice.

[0022] 2) Preparation of bacterial cellulose from jujube juice

[0023] The above-mentioned jujube juice is directly used as a medium for cultivating bacterial cellulose, 50ml is taken, and 2% glucose, 0.1% ammonium citrate, 0.5% Na 2 HPO 4 and 0.1% L-histidine to form a medium, adjust the pH value of the medium to 6.0, and then use a high-pressure steam sterilizer to cook at 121° C. for 20 minutes. Finally, 10% culture solution of Acetobacter xylinum was added under aseptic conditions, and cultured statically at 30° C. for 6 days to obtain bacterial cellulose membrane.

[0024] The weight of the obtained bacterial cellulose wet film is 23.440g / 50ml.

Embodiment 2

[0026] 1) Extraction of jujube juice

[0027] Put 100g of red dates in 1000L of water, boil at 100°C for 20 minutes, let it cool down, and filter through a sieve to obtain jujube juice.

[0028] 2) Preparation of bacterial cellulose from jujube juice

[0029] The above-mentioned jujube juice is directly used as a medium for cultivating bacterial cellulose, 50ml is taken, and 2% glucose, 0.1% ammonium citrate, 0.5% Na 2 HPO 4 and 0.1% L-arginine to prepare a medium, adjust the pH value of the medium to 6.0, and then use a high-pressure steam sterilizer to cook at 121° C. for 20 minutes. Finally, 10% culture solution of Acetobacter xylinum was added under aseptic conditions, and cultured statically at 30° C. for 6 days to obtain bacterial cellulose membrane.

[0030] The weight of the obtained bacterial cellulose wet film is 16.809g / 50ml.

Embodiment 3

[0032] 1) Extraction of jujube juice

[0033] Put 100g of red dates in 1000L of water, boil at 100°C for 20 minutes, let it cool down, and filter through a sieve to obtain jujube juice.

[0034] 2) Preparation of bacterial cellulose from jujube juice

[0035] The above-mentioned jujube juice is directly used as a medium for cultivating bacterial cellulose, 50ml is taken, and 2% glucose, 0.1% ammonium citrate, 0.5% Na 2 HPO 4 and 0.1% L-serine to prepare a medium, adjust the pH value of the medium to 6.0, and then use a high-pressure steam sterilizer to cook at 121° C. for 20 minutes. Finally, 10% culture solution of Acetobacter xylinum was added under aseptic conditions, and cultured statically at 30° C. for 6 days to obtain bacterial cellulose membrane.

[0036] The weight of the obtained bacterial cellulose wet film is 15.619g / 50ml.

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Abstract

The invention discloses a method of comprehensively preparing bacterial cellulose from waste liquor of candied date. The bacterial cellulose is used to produce textiles, medical dressing, and filtering materials. The method comprises the following steps: directly using waste liquor of candied date as the culture medium to culture bacterial cellulose, adding the following components: glucose, ammonium citrate, Na2HPO4, and amino acid, which respectively account for 2%, 0.1%, 0.5%, and 0.01-0.1% of the weight of waste liquor of candied date to prepare the culture medium; sterilizing the culture medium, adding 6 to 10% of Gluconacetobacter xylinum CGMCC No.2955 culture liquid, allowing the culture medium to stand still, and culturing for 5 to 10 days at a temperature of 25 to 30 DEG C to obtain the bacterial cellulose. The invention provides a novel method for comprehensively utilizing waste food. At the same time, the production cost of bacterial cellulose is reduced, the biological resources are fully utilized, the raw material are cheap and easily available, the generation of wastes is reduced, and more economic benefits are generated.

Description

technical field [0001] The invention relates to a method for comprehensive utilization of food waste, in particular to a method for producing nano-scale bacterial cellulose from jujube juice waste for use in textiles, medical dressings, and filter materials. Background technique [0002] Bacterial cellulose (Bacterial Cellulose, BC) was discovered by Adrian Brown in 1886 when cultivating Acetobacter. Bacterial cellulose is a high-molecular polymer composed of glucose linked by β-1,4 glycosidic bonds. It has many characteristics superior to plant cellulose, such as no hemicellulose, lignin, and pectin. In addition, bacterial cellulose also has some advantages: (1) good biocompatibility and biodegradability; (2) high water holding capacity and high air permeability; (3) high crystallinity, polymerization degree and very Consistent molecular orientation; (4) high tensile strength and elastic modulus; (5) biosynthesis can be regulated. Due to the excellent properties of bacter...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C12R1/01
Inventor 李政汪丽粉张健飞巩继贤贾士儒
Owner TIANJIN POLYTECHNIC UNIV
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