Method for enzymatic synthesis of L-ascorbyl palmitate combined with stirring method under ultrasonic conditions

A technology of ascorbyl palmitate and ascorbic acid, applied in the field of food additive synthesis and processing, can solve the problems of cumbersome operation, low utilization rate, time-consuming and labor-intensive, etc., and achieves the effects of simple operation, improved efficiency, and reduced damage

Active Publication Date: 2019-04-23
GUANGZHOU FOOD IND RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At the same time, for the conventional production process, lipase and molecular sieve basically sink to the bottom of the container during the reaction, and the utilization rate is not high. After the system reaction is completed, lipase and molecular sieve need to be recovered through two or even multiple filtrations. use, the operation is very cumbersome, time-consuming and labor-intensive

Method used

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  • Method for enzymatic synthesis of L-ascorbyl palmitate combined with stirring method under ultrasonic conditions
  • Method for enzymatic synthesis of L-ascorbyl palmitate combined with stirring method under ultrasonic conditions
  • Method for enzymatic synthesis of L-ascorbyl palmitate combined with stirring method under ultrasonic conditions

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Effect test

Embodiment 1

[0032] (1) 15g 4A type molecular sieves are separately installed in the filter; 150ml tert-butanol is added to a closed reaction vessel with a stopper, then 3g L-ascorbic acid and 0.6g Novozymes 435 type ( 435) Lipase, put it under the condition of ultrasonic (ultrasonic power is 140W), start the mixer (rotating speed is 200r / min), after L-ascorbic acid dissolves, add 12g palmitic acid, 55 ℃ water bath constant temperature reaction 3h, 4h, 5h or 6h; the reaction process is to use a circulation pump to carry out uninterrupted circulation of the reaction system between the filter and the reaction vessel. During circulation, the reaction liquid enters from the bottom of the reaction vessel, flows out from the upper feeding port of the reaction vessel, and enters the circulation pipeline from the reaction vessel. Add an 80-mesh screen at the outlet of the lipase to prevent lipase from flowing into the circulation pipeline;

[0033] (2) removing lipase by filtration, the obtained ...

Embodiment 2

[0040] (1) 15g 4A type molecular sieves are separately installed in the filter; 150ml tert-butanol is added to a closed reaction vessel with a stopper, followed by 3g L-ascorbic acid, 1.0g phytic acid and 0.6g Novozymes 435 type ( 435) Lipase, put it under the condition of ultrasonic (ultrasonic power is 140W), start the mixer (rotating speed is 200r / min), after L-ascorbic acid dissolves, add 12g of palmitic acid, 55 ℃ water bath constant temperature reaction for 6h; the reaction process is to use The circulation pump performs uninterrupted circulation of the reaction system between the filter and the reaction vessel. During circulation, the reaction liquid enters from the bottom of the reaction vessel and flows out from the upper feeding port of the reaction vessel. Add 80 mesh Screen to prevent lipase from flowing into the circulation pipeline;

[0041] (2) removing lipase by filtration, the obtained filtrate was distilled off under reduced pressure at 55° C. to remove the ...

Embodiment 3

[0049] (1) 15g 4A type molecular sieves are separately installed in the filter; 150ml tert-butanol is added to a closed reaction vessel with a stopper, followed by 3g L-ascorbic acid, 1.0g phytic acid and 0.6g Novozymes 435 type ( 435) Lipase, put it under the condition of ultrasonic (ultrasonic power is 140W), start the mixer (rotating speed is 200r / min), after L-ascorbic acid dissolves, add 12g of palmitic acid, 55 ℃ water bath constant temperature reaction for 6h; the reaction process is to use The circulation pump performs uninterrupted circulation of the reaction system between the filter and the reaction vessel. During circulation, the reaction liquid enters from the bottom of the reaction vessel and flows out from the upper feeding port of the reaction vessel. Add 80 mesh Screen to prevent lipase from flowing into the circulation pipeline;

[0050] (2) removing lipase by filtration, the obtained filtrate was distilled off under reduced pressure at 55° C. to remove the ...

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Abstract

The invention belongs to the technical field of synthesis and processing of food additives and discloses a method for enzymatic synthesis of L-AP (L-ascorbyl palmitate) under an ultrasonic condition in combination with a stirring method. According to the method, a 4A type molecular sieve is independently mounted in a filter; tertiary butanol is added to an airtight reaction container with a plug, L-ascorbic acid, phytic acid and novozymes 435 type lipase are added, the mixture is put under an ultrasonic condition, a stirring machine is started, palmitic acid is added after the mixture is dissolved, and the mixture has a water-bath thermostatic reaction; filtrate obtained through suction filtration is distilled under reduced pressure; an obtained solid is dissolved with a methylbenzene and ethyl acetate mixed solvent with the mass ratio being 1:2 and then washed with water, a water layer is discharged through a liquid separation funnel, the remaining substances are left to stand for 4 hours, and suction filtration is performed; a finished product is obtained through vacuum drying. The reaction time can be remarkably shortened, a purification solvent is changed, purification is performed once instead of twice, the efficiency is improved, the energy consumption and the loss are reduced, the number of times for repeated use of a catalyst enzyme is increased to 12 from original 8, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of food additive synthesis and processing, and in particular relates to a method for synthesizing L-ascorbyl palmitate under ultrasonic conditions combined with stirring method and enzyme catalysis. Background technique [0002] L-ascorbyl palmitate (L-AP), also known as Vc ester, English abbreviation L-AP, white or yellow-white crystalline powder, soluble in alcohol, ether and oil. L-AP is a non-toxic and harmless multifunctional nutritional anti-oxidant preservative. It has anti-oxidation and nutritional enhancement functions and high temperature resistance. It is suitable for medicine, health care products, cosmetics, etc., and is suitable for baking and frying. Antioxidant of oil, often used in oily foods, edible oils, animal and vegetable oils and high-grade cosmetics, and can also be used in various infant foods and milk powder. At present, industrial production adopts chemical synthesis method, which...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
CPCC12P7/6436C12P17/04
Inventor 何金银曾永青李善军吴国城
Owner GUANGZHOU FOOD IND RES INST
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