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Acinetobacter pitae and its uses

A bacillus and strain technology, applied in the field of microorganisms, can solve the problems that need to be improved, the large-scale industrialization and application of biological treatment technology is difficult, and the ability of microorganisms to degrade oil is poor.

Active Publication Date: 2019-05-31
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, the existing petroleum wastewater biological treatment technology generally has problems such as poor microbial oil degradation ability, poor adaptability of bacterial flora, frequent inoculation, etc., resulting in high operating costs, difficulty in continuous operation, and difficulty in reinjection of residual oil content after treatment. Water quality standards make it difficult to realize large-scale industrial application of biological treatment technology
[0005] Therefore, the current technology related to the biological treatment of petroleum wastewater still needs to be improved.

Method used

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  • Acinetobacter pitae and its uses
  • Acinetobacter pitae and its uses
  • Acinetobacter pitae and its uses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1: Screening of Acinetobacter pittii SYJ1-3 with petroleum degrading ability

[0031] Screening steps: Take 500g of soil samples from 10 long-term oil-contaminated soils at 5-10cm below the surface of Changqing Oilfield. After mixing evenly, take 10g of the mixed soil samples and add them to 250mL of LB medium Microbial enrichment was carried out in shake flasks, specifically cultured on a shaker at 28-30°C and 180rpm for 5 days to obtain the enrichment solution, sucked 1 mL of the above-mentioned enrichment solution and transferred it to fresh LB medium, and continued the above For microbial enrichment, absorb 1 mL of the enriched solution obtained after culturing for 5 days and transfer it to an inorganic salt medium with petroleum as the only carbon source (the formula is: NH 4 NO 3 (2g / L), Na 2 HPO 4 12H 2 O (3.8g / L), KH 2 PO 4 (1.5g / L), NaCl(1g / L), MgSO 4 (0.2g / L), FeSO 4 ·7H 2 O0.05g / L, anhydrous CaCl 2 0.05g / L, crude oil (5g / L), pH 7.0-7.2, and...

Embodiment 2

[0035] Embodiment 2: Laboratory shake flask experiment measures the effect of Acinetobacter pittii SYJ1-3 degrading petroleum

[0036] From the Acinetobacter pittii SYJ 1-3 purification plate obtained in Example 1, select a ring of bacterium colonies to insert into LB medium, the amount of liquid in the shake flask is 30% of the volume of the shake flask, the culture temperature is 28~30°C, and the culture speed is 150- 200rpm / min, the cultivation time is 10-16h, and the SYJ1-3 seed culture solution is obtained. Weigh 100mg of petroleum and add it to a shake flask containing 90ml of inorganic salt medium, then inoculate 10ml of the SYJ1-3 seed culture solution obtained above, and use the petroleum-containing inorganic salt medium without inoculation of SYJ1-3 seed culture solution as a control The initial petroleum concentration in the solutions of the experimental group and the control group was 1 mg / mL. The shake flasks of the above experimental group (containing SYJ1-3 see...

Embodiment 3

[0038] Embodiment 3: Application and environmental adaptability test of Acinetobacter pittii SYJ1-3 in small test SBR device

[0039] The influent water used in this experiment is artificially prepared simulated wastewater and raw water produced from Changqing Oilfield. The artificially prepared simulated wastewater formula is mainly composed of NH 4 Cl(0.02g / L), K 2 HPO 4 , NaCl (2g / L), NaHCO 3 (4g / L), Na 2 CO 3 (1g / L), MgSO 4 (0.2g / L), Fe 2 SO 4 ·7H 2 O (0.05g / L), anhydrous CaCl 2 (0.05g / L), glucose (0.1g), petroleum (50-500mg / L), pH (7.5-8.5). The sludge inoculated in the SBR when the reactor was started was taken from the activated sludge of a sewage treatment plant. The amount of sludge inoculum was 1530mg / L. When the SBR process runs to the 15th day, the sludge concentration drops to 390mg / L, and the Acinetobacter pittii SYJ1-3 bacterial strain of the enlarged culture is added into the SBR process, and the dosage ratio is about 10% (volume percentage). The SBR ...

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Abstract

The invention discloses Acinetobacter piterii and its application, wherein, the Acinetobacter piteter was preserved in the General Microbiology Center (CGMCC) of China Microbiological Culture Collection Management Committee (CGMCC) on November 16, 2015, and the address of the preservation unit is Chaoyang, Beijing Institute of Microbiology, Chinese Academy of Sciences, No. 1, Yard 1, Beichen West Road, District, the preservation number is CGMCC NO.11670, and the preservation name is Acinetobacter pittii SYJ1‑3. The Acinetobacter piterii of the present invention can grow and proliferate with petroleum as a carbon source, utilize its own metabolism to degrade petroleum, and can be effectively used in the treatment of petroleum wastewater, petroleum-contaminated soil, etc., not only has high petroleum degradation efficiency, but also is simple and convenient to operate and low in cost. lower.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to Acinetobacter piterii and its use, and more specifically to Acinetobacter piteri and its use in degrading petroleum, and a method for degrading petroleum. Background technique [0002] With the increasing demand for energy due to economic development, most oilfields in my country have now entered the secondary / tertiary oil recovery period, and the difficulty of mining has increased. As a result, improving oil recovery by water injection or changing the characteristics of injected water has become a major issue in most oilfields. mining method. Every year, a large amount of clean water with good compatibility with underground reservoirs is injected into the target layer, and at the same time, a large amount of oily wastewater is extracted from the target layer along with the production fluid. The comprehensive water content of the production fluid of most onshore oilfields in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/32
CPCB09C1/10C02F3/344C02F2101/32C12N1/205C12R2001/01
Inventor 左剑恶李晓霞
Owner TSINGHUA UNIV
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