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A method and device for biodegrading sulfamethoxazole

A technology of sulfamethoxazole and biodegradation, applied in the field of environmental pollution control, can solve problems such as poor ability, and achieve the effect of promoting biomass and inhibiting re-synthesis

Inactive Publication Date: 2018-02-13
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in previous studies, it was found that the ability of Alcaligenes faecalis and Pseudomonassp.denitrificans to degrade SMX was not as good as that of Alcaligenes faecalis alone
The research on the degradation of SMX by mixed bacteria in membrane bioreactor has not been reported yet

Method used

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  • A method and device for biodegrading sulfamethoxazole
  • A method and device for biodegrading sulfamethoxazole
  • A method and device for biodegrading sulfamethoxazole

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A method for biodegrading sulfamethoxazole, comprising the steps of:

[0028] (1) Seed culture: put Alcaligenes faecalis (Alcaligenes faecalis) stored at -80°C with the preservation number CGMCC No.1.767 into 50mL seed culture medium, and cultivate it for 24h at a temperature of 30°C and a rotation speed of 220rpm. , to obtain the primary seeds of Alcaligenes faecalis; insert 1 mL of the primary seeds of Alcaligenes faecalis into 100 mL of new seed medium at a temperature of 30°C and a rotation speed of 220rpm, and cultivate for 24 hours to obtain Alcaligenes faecalis the secondary seed of

[0029]Put Pseudomonassp.denitrificans stored at -80°C with the preservation number ATCC 13867 into 50mL seed culture medium, and cultivate them for 24 hours at a temperature of 30°C and a rotation speed of 220rpm to obtain pseudomonas denitrificans. First-class seeds of Bacillus denitrifica; insert 1mL of first-class seeds of Pseudomonas denitrification into 100mL of new seed mediu...

Embodiment 2

[0041] A method for biodegrading sulfamethoxazole, comprising the steps of:

[0042] (1) (1) with embodiment 1;

[0043] (2) Degradation solution preparation: 3L sulfamethoxazole production discharge wastewater (sulfamethoxazole concentration is 65mg / L) was mixed with 0.6L basic inorganic salt culture medium to make degradation solution;

[0044] (3) The degradation solution is loaded into the first fermentor 1 and the second fermenter 2 respectively; the secondary seeds of Alcaligenes faecalis 540 The inoculum size of 0.2 was inserted into the first fermentor 1 equipped with 3L degradation liquid, and the secondary seeds of Pseudomonas denitrifica were pressed by the initial OD 540 The inoculation amount of 0.2 was connected to the second fermenter 2 equipped with 3L of degradation solution, and the air volume was 0.5vvm, the stirring speed was 300rpm, and the temperature was 30°C, respectively, for 14h;

[0045] (4) Pump the fermented liquid in the first fermentation tank ...

Embodiment 3

[0049] A method for biodegrading sulfamethoxazole, comprising the steps of:

[0050] (1) (1) with embodiment 1;

[0051] (2) Degradation solution preparation: 3.6L sulfamethoxazole production discharge wastewater (sulfamethoxazole concentration is 65mg / L) is mixed with 0.6L basic inorganic salt culture medium to make degradation solution;

[0052] (3) The degradation solution is loaded into the first fermentor 1 and the second fermenter 2 respectively; the secondary seeds of Alcaligenes faecalis 540 The inoculum size of 0.2 was inserted into the first fermentor 1 equipped with 3L degradation liquid, and the secondary seeds of Pseudomonas denitrifica were pressed by the initial OD 540 The inoculation amount of 0.2 was connected to the second fermenter 2 equipped with 3L of degradation liquid, and the air volume was 0.5vvm, the stirring speed was 300rpm, and the temperature was 30°C, respectively, for 16h;

[0053] (4) Pump the fermented liquid in the first fermentor into the ...

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Abstract

The invention discloses a method and a device for biologically degrading SMX (sulfamethoxazole). The method comprises steps as follows: (1) performing seed culture to obtain secondary seeds of alcaligenes faecalis and pseudomonas sp. denitrificans; (2) preparing a degradation liquid; (3) containing the degradation liquid into two fermentation tanks, and inoculating two fermentation tanks with secondary seeds of alcaligenes faecalis and pseudomonas sp. denitrificans for fermentation; (4) filtering two fermentation broths with a hollow fiber membrane module, exchanging the fermentation tanks for fermentation filtrates for 2-3 h continuously, and storing the fermentation filtrates into two storage tanks respectively; (5) washing filtered alcaligenes faecalis back into respective fermentation tanks for fermentation. According to the device and the method, two bacterial cells are not contacted, so that low SMX degradation rates caused by inhibition of biomass due to contact of two bacterial cells are avoided. Meanwhile, the hollow fiber membrane system can promote the biomass of the two bacteria and inhibit re-synthesis of SMX.

Description

technical field [0001] The invention belongs to the field of environmental pollution control, and relates to a method and a device for biodegrading sulfamethoxazole. Background technique [0002] Sulfamethoxazole (SMX) is one of the commonly prescribed, consumed sulfa antibiotics. The concentration of SMX can be detected in wastewater as high as mg / L level, the content in surface water is ng / L level, and even 410ng / L is detected in groundwater. Its existence in the aquatic environment can lead to severe water pollution, threatening the survival of aquatic plants, animals and microorganisms. Studies have found that SMX can change the composition of microbial flora, lead to the spread of resistance genes, and have mutagenicity or phytotoxicity to aquatic organisms. Many literatures have reported that biodegradation is the most important way to eliminate SMX in sewage treatment. The efficiency of its elimination has been studied and some main strains with SMX degradation abil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C02F3/34C12N1/20C02F103/36C12R1/05C12R1/38
CPCC02F3/34C02F3/341C02F2101/38C02F2101/40C02F2103/343C02F2103/36C02F2305/06C12N1/20
Inventor 程景胜周娇元英进张艺碧
Owner TIANJIN UNIV
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