A kind of injectable bone graft material and preparation method thereof

Abstract

The invention relates to the technical field of medical bone repair materials, in particular to an injectable bone grafting material, a preparation method and a use method. The bone grafting material is characterized by being prepared from, by weight, 6-10 parts of decalcified bone matrix, 0.8-1.5 parts of PRP, 0.5-2 parts of autologous hemocyte growth factors, 0.5-2 parts of Radix Salviae Miltiorrhizae injection, 0.05-0.2 part of doxycycline and 0.001-0.005 part of scopolamine, decalcified bone matrix, PRP, autologous hemocyte growth factors, doxycycline and scopolamine are mixed in real time during surgery, the mixture is arranged in a 10 ml syringe and compounded into an injectable mixture, and the bone grafting material is injected in an intraoperative bone defect part or the part (such as an intervertebral space) needing bone fusion enhancement, and has the advantages of being good in union effect, resistant to infection, high in osteogenic induction capacity, high in osteogenic strength, and the like.

Description

technical field [0001] The invention relates to the technical field of medical bone repair materials, in particular to an injectable bone graft material with good healing effect, anti-infection, strong osteogenic induction ability and high osteogenic strength, and its preparation method and use method. Background technique [0002] As we all know, bone transplantation is the main method to treat bone defects, and it is also one of the most commonly used treatment methods in orthopedics. The traditional graft material is autologous bone, which has the characteristics of strong bone formation and easy healing, but due to limited sources and secondary injuries , increase the chance of infection and other reasons limit its clinical application; allograft bone does not have its own osteogenic ability, and the healing mainly depends on osteoconduction and osteoinduction, and the antigenicity of grafted bone is an important factor affecting the healing of allograft bone and the host...

AI Technical Summary

Problems solved by technology

[0002] As we all know, bone transplantation is the main method to treat bone defects, and it is also one of the most commonly used treatment methods in orthopedics. The traditional graft material is autologous bone, which has the characteristics of strong bone formation and easy healing, but due to limited sources and secondary injuries , increase the chance of infection and other reasons limit its clinical application; allograft bone does not have its own osteogenic ability, and the healing mainly depends on osteoconduction and osteoinduction, and the antigenicity of grafted bone is an important factor affecting the healing of allograft bone and the host The main obstacle of xenograft bone is immune rejection; artificial bone has a single osteogenesis method, incomplete repair, slow healing process, and lack of biological activity. At present, bone graft materials are generally used for treatment. There are two main stages, hematoma organization stage (2 weeks), which is mainly platelet aggregation and activation, granulocyte and macrophage migration; primitive callus formation stage (3-8 weeks), which is the proliferation, differentiation and reproduction of mesenchymal cells stage; plastic stage (8-12 weeks), the reconstruction of various tissue cells, platelet aggregation in the hematoma organization stage starts the blood coagulation reaction, there are two ways to complete the whole process, one is repair, the other is regeneration

The repair process is that when the tissue damage exceeds a certain limit, the proliferation and differentiation of mesenchymal cells cannot meet the needs of tissue regeneration. At this time, the proliferation and differentiation of fibroblasts replace mesenchymal cells to repair tissue wounds and regenerate. The process is that there are sufficient proliferation and differentiation of mesenchymal cells in the tissue injury area, and the function and activity of various tissues and organs are rebuilt. Tissue reconstruction is an ideal healing approach. In the process of tissue healing, platelets are the main regulators of the inflammatory phase. It plays an important role in the stage of cell reproduction and differentiation, and is the key component of the successful tissue reconstruction pathway. At present, the bone graft materials used in bone grafting have disadvantages such as poor healing effect, easy infection, and slow induction of osteogenesis.