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Application of AI-2 in improving bacteria acid resistance and/or oxygen resistance and method for improving bacteria acid resistance and/or oxygen resistance

An AI-2, acid-resistant technology, applied in the field of microorganisms, can solve the problems of poor taste, unstable genetic characteristics of bifidobacteria, and complicated operation.

Active Publication Date: 2016-06-01
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the coating process is cumbersome, and the taste of yogurt or lactic acid beverages added with coated strains will deteriorate, and the genetic characteristics of acid-resistant and domesticated bifidobacteria are also relatively unstable.
[0004] In addition, bifidobacteria are anaerobic bacteria, their growth will be significantly inhibited in a micro-aerobic environment, and complete exposure to oxygen will lead to their complete inactivation

Method used

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  • Application of AI-2 in improving bacteria acid resistance and/or oxygen resistance and method for improving bacteria acid resistance and/or oxygen resistance
  • Application of AI-2 in improving bacteria acid resistance and/or oxygen resistance and method for improving bacteria acid resistance and/or oxygen resistance
  • Application of AI-2 in improving bacteria acid resistance and/or oxygen resistance and method for improving bacteria acid resistance and/or oxygen resistance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] The effect of formula (1) compound on the acid resistance of bifidobacteria during acid stress

[0046] (1) Verification of acid stress

[0047] Inoculate 1% in the MRS liquid culture medium from CGMCCNo.2265 (BBMN68) seed culture liquid, 37 ℃ of anaerobic cultures, reach mid-logarithmic phase (about 6h) and get bacterial liquid sample, measure OD600 (reached) with ultraviolet-visible spectrophotometer 0.6 or more), divide the bacterial liquid into two parts A, B, C and D, and centrifuge at 8000rpm for 5min; discard the supernatant, and use MRS with pH6.5, pH4.0, pH2.5 and pH1 respectively Wash bacterial cells A, B, C, and D with liquid medium, centrifuge again: 8000rpm, 5min; discard the supernatant, and resuspend the precipitates A, B, C, and D in equal volumes of pH 6.5 and pH 4.0 respectively. , MRS liquid medium of pH 2.5 and pH 1, anaerobic culture at 37°C, take A, B, C, and D bacteria liquids at 1h, 2h, 3h, and 4h respectively, dilute 10 times with the diluent, ...

Embodiment 2

[0056] When being used for illustrating acid stress, the compound shown in formula (1) is to the impact of bifidobacterium acid resistance

[0057] The acid stress experiment of the pH4 group was carried out according to the method of Example 1, except that the bifidobacterium used was Bifidobacterium juvenile CGMCC No.6270.

[0058] The results showed that with the prolongation of the culture time, the number of survivors in the pH4.0 control group and the groups added with different concentrations of the compound of formula (1) decreased gradually. At each time point, the number of survivors who added different concentrations of the formula (1) compound group was significantly higher than that of the pH4.0 control group (such as image 3 Shown), but the degree of improvement is significantly lower than in Example 1. However, there was no significant difference in the number of survivors among groups of compounds of the same different concentrations of formula (1).

[0059]...

Embodiment 3

[0066] Effect of compounds shown in formula (1) on oxygen tolerance of bifidobacteria during oxygen stress

[0067] (1) Verification of oxygen stress

[0068] Inoculate 1% of the BBMN68 seed culture solution into the MRS liquid medium, culture anaerobically at 37°C, and inoculate the bacterial solution into the MRS medium with different concentrations of oxygen according to the inoculation amount of 1% after activation for one generation, respectively B: Filled with N2, does not contain CYS (dissolved oxygen is about 0.4mg / L), C: contains 0.05% by weight of CYS, does not fill with N2 (corresponding oxygen concentration is 0.5mg / L) and D: does not fill with N2 , also does not contain CYS (the corresponding oxygen concentration is 2mg / L), strictly anaerobic (A: containing the concentration of 0.05% by weight of CYS and containing the concentration of 99% by volume of N 2 ). Cultivate at 37°C, measure OD600 with a UV-visible spectrophotometer every 2 hours until 12 hours, the r...

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Abstract

The invention relates to the microorganism field and discloses application of bacteria quorum sensing signal molecule AI-2 in improving bacteria acid resistance and / or oxygen resistance, and further discloses a method for improving the bacteria acid resistance and / or oxygen resistance. The method comprises the step that a compound of a structure shown by the formula (1) is added in the process that bacteria bear acid stress and / or oxygen stress. By the aid of the technical scheme, the compound of the structure shown by the formula (1) is added in the process that bacteria bear the acid stress and / or oxygen stress, and the compound can be converted into the bacteria quorum sensing signal molecule AI-2 in the bacteria growth environment, so that the influences of the acid stress and / or oxygen stress on bacteria can be reduced to the minimum.

Description

technical field [0001] The present invention relates to the field of microorganisms, in particular to the application of a bacterial quorum sensing signal molecule AI-2 in improving the acid resistance and / or oxygen resistance of bacteria, and a method for improving the acid resistance and / or oxygen resistance of bacteria . Background technique [0002] Bifidobacteria are the most beneficial flora in the intestine, and the suitable pH value for growth is 6.5-7.5. The reduction or even disappearance of the number of bifidobacteria is a sign of an "unhealthy" state. Bifidobacteria are a barometer of human health. The main way to replenish bifidobacteria is to drink yogurt or beverages containing bifidobacteria, or directly drink the probiotics of bifidobacteria (bacteria powder or 1:3 serving liquid). [0003] It is known that the acidity of human gastric juice is generally 0.9-1.5. Under normal circumstances, the survival rate of bifidobacteria will be greatly reduced after ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/38C12N1/20
CPCC12N1/20C12N1/38
Inventor 任发政金君华秦倩刘松玲赵亮郭慧媛
Owner CHINA AGRI UNIV
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