Nucleic acid structure of which interchain exchange is achieved by support DNA (deoxyribonucleic acid) and synthesis method thereof

Abstract

The invention discloses a nucleic acid structure of which the interchain exchange is achieved by a support DNA (deoxyribonucleic acid). The nucleic acid structure is a two-dimensional or three-dimensional nucleic acid structure with controllable size and shape, which is formed by achieving interchain exchange by the support DNA. The nucleic acid structure comprises a set of parallel double helices connected by interchain exchange and a single-chain DNA segment, wherein the double helices are formed by carrying out folding and interchain exchange by the support DNA, carrying out specific matching with a plurality of short-chain DNAs and carrying out annealing reaction; and the single-chain DNA is the part of the support DNA which does not form the double helices. The invention provides the multiple nucleic acid structures and synthesis methods thereof. The nucleic acid structure is a variant technique based on DNA paper folding. The invention aims to form the nucleic acid structure with controllable size, shape, complexity and modification by achieving interchain exchange by using the support DNA. The nucleic acid structure is completed by the support DNA / short-chain DNA self-assembly process, and has the advantages of higher yield and simple design; and the predicated two-dimensional and three-dimensional structures can be simultaneously synthesized.

Description

technical field [0001] The invention relates to a nucleic acid structure and a synthesis method thereof in the field of DNA nanotechnology. More specifically, it relates to methods for synthesizing two-dimensional and three-dimensional nucleic acid structures with controllable size, shape, and complexity at the nanoscale using strand exchange achieved by scaffold DNA, and the nucleic acid structures themselves. Based on the addressability of each short strand of DNA in the resulting structure, nucleic acid structures with specific cavities and modifications can be obtained. Background technique [0002] In the 1980s, Seeman first proposed that DNA could be assembled into a complex spatial structure using the principle of DNA base complementary pairing, creating a new field of using DNA as a nanoscale construction material rather than a carrier of genetic information, and named it as DNA nanotechnology. Subsequently, researchers constructed different primitive modules, such...

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Problems solved by technology

Subsequently, researchers constructed different primitive modules, such as DX (double-crossover), TX (triple-crossover) modules, cross modules and symmetrical modules, and assembled them to obtain a variety of graphic structures (two-dimensional arrays, square grid, etc.), but the modular assembly is based on the complementary base pairing of small structural units to form a larger graphic structure, and its size and shape are difficult to precisely control

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