Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of phosphorylated epimedium polysaccharide

A technology of Epimedium polysaccharide and phosphorylation, which can be applied in the direction of antitoxic agent, antiviral agent, drug combination, etc., can solve problems such as the blank of activity research.

Active Publication Date: 2016-05-25
NANJING AGRICULTURAL UNIVERSITY
View PDF6 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the current studies on epimedium polysaccharide derivatives focus on sulfation and selenization, and the research on phosphorylated epimedium polysaccharides has not been reported, and its activities in immune regulation, antiviral and antioxidant also blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of phosphorylated epimedium polysaccharide
  • Preparation method of phosphorylated epimedium polysaccharide
  • Preparation method of phosphorylated epimedium polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Embodiment one: the preparation of epimedium polysaccharide

[0010] Take 1000g of Epimedium herba that has been dried and crushed at 60°C, add enough 95% ethanol, heat and reflux for 3 times to degrease, take it out, dry, add 10 times the volume of the dregs to decoct twice, 3 hours each time, Filtrate, decoct the dregs with 8 times the volume of water once, decoct for 2 hours, filter, combine the filtrates for 3 times, concentrate to 1000mL, and centrifuge to remove impurities. Add 95% ethanol to the supernatant until the volume fraction of ethanol is 80%, let stand at 4°C for 12h, centrifuge at 3000rpm for 20min, collect the precipitate, dry it in vacuum, add water to redissolve, add 95% ethanol until the volume fraction of ethanol is 80%, and set aside at 4°C Let stand for 12 hours, centrifuge at 4000rpm for 15 minutes, collect the precipitate, and freeze-dry to obtain Epimedium crude polysaccharide. Accurately weigh the epimedium crude polysaccharide, add 10 times...

Embodiment 2

[0011] Example 2: Treatment of Phosphorylated Epimedium Polysaccharide and Determination of Phosphate Content

[0012] Drawing of phosphate standard curve:

[0013] Preparation of phosphate standard solution: Accurately weigh 3.6g tris and 120mgMgCl 2 ·6H 2 O was dissolved in distilled water, and diluted with water to 300ml, and finally adjusted to pH 7 with 1mol / L HCl, as a Tris buffer solution. Take equal volumes of 20% ascorbic acid aqueous solution (m / m), 3mol / L H 2 SO 4 Solution and 3% ammonium molybdate solution (m / m) mixture, as phosphorus determination reagent. Precisely weigh 0.7165g of potassium dihydrogen phosphate dried to constant weight and dissolve it in deionized water, transfer it into a 1000ml volumetric flask, add deionized water to make it up to the mark, and shake well. Draw 1mL of this solution, dilute it with deionized water in a 50mL volumetric flask and shake well, as a phosphate standard solution.

[0014] Preparation of Tris buffer solution: Ac...

Embodiment 3

[0020] Example 3: Single Factor Test of Phosphorylated Epimedium Polysaccharide

[0021] Screening test of phosphorylation reagent: Accurately weigh the purified epimedium polysaccharide, and prepare a 10 mg / mL solution with deionized water. According to the proportions in Table 1, the mixed reagents of sodium tripolyphosphate and sodium trimetaphosphate in different mass ratios were used as phosphorylation reagents, and an equal mass of 5% Na 2 SO 4 , reacting for 2 hours under the conditions of reaction pH value 6.0 and reaction temperature 20° C., to phosphorylate Epimedium polysaccharide. Modified products with a molecular weight cut-off of 3.5×10 3 The dialysis bag was dialyzed with deionized water for 48 hours, and then freeze-dried to obtain phosphorylated epimedium polysaccharide. The content of phosphate in the modified product was determined by the method of Example 2, and the influence of the ratio of phosphorylation reagent on the amount of grafted phosphate of ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of preparation and structural transformation of traditional Chinese medicine polysaccharide and relates to a preparation method of phosphorylated epimedium polysaccharide. The preparation method of the phosphorylated epimedium polysaccharide comprises the following steps: carrying out heating reaction on a phosphorylating reagent (sodium tripolyphosphate-sodium trimetaphosphate), epimedium polysaccharide and sodium sulfate for a period of time under alkaline condition, carrying out phosphorylation molecular modification, and selecting out optimal conditions for phosphorylation modification on the epimedium polysaccharide by carrying out a response surface experiment with content of phosphate radicals of products and reagent ratio, reaction temperature, reaction pH value and reaction time as factors. After the phosphorylated epimedium polysaccharide is prepared, in vitro activity of a modified product is studied, and results show that the phosphorylated epimedium polysaccharide has a certain immunoregulation, antiviral and antioxidant effects.

Description

technical background [0001] The invention belongs to the technical field of preparation and structural transformation of traditional Chinese medicine polysaccharides, and relates to a preparation method of phosphorylated epimedium polysaccharide (Phosphorylated Epimedium Polysaccharide, PEP). Background technique [0002] Polysaccharides are an important class of macromolecular substances in organisms. They have pharmacological activities such as immune regulation, anti-virus, anti-tumor, blood sugar regulation, anti-aging, anti-oxidation, anti-inflammatory, antibacterial, and liver protection. They are widely used in clinical practice. Studies have shown that chemical modification of polysaccharides such as methylation, acetylation, sulfation, phosphorylation, and selenization can not only change the physical and chemical properties of polysaccharides, but also enhance the pharmacological activity of polysaccharides. For example, sulfated astragalus polysaccharides can sig...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C08B37/00A61P37/02A61P39/06A61P31/12
CPCC08B37/00
Inventor 武毅倪海钰吴秋月倪涵刘家国王德云胡元亮刘伟新廖智
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com