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Microbial agent for preventing and controlling solanaceous vegetable virosis and preparation method thereof

A technology of microorganisms and bacterial agents, which is applied in the field of bacillus composite microbial bacterial agents and its preparation, and the field of Trichoderma to prevent and control viral diseases of solanaceous vegetables. It can solve the problems of drug resistance, environmental pollution, pesticide residues, etc., and reach the control range Increase, control effect improvement, obvious inducement and growth promotion effects

Inactive Publication Date: 2016-05-11
텐진인스티튜트오브플랜트프로텍션
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Problems solved by technology

[0006] The present invention aims to provide a preparation of Trichoderma amyloliquefaciens and Bacillus amyloliquefaciens for preventing and controlling viral diseases of solanaceous vegetables and a preparation method thereof, which can be used for preventing and controlling viral diseases of solanaceous vegetables and solve the problems caused by chemical pesticide control. Pesticide Residues, Drug Resistance and Environmental Pollution Issues

Method used

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  • Microbial agent for preventing and controlling solanaceous vegetable virosis and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0046] Isolation and screening of Trichoderma dark green and Bacillus amyloliquefaciens.

[0047] Trichoderma aureus and Bacillus amyloliquefaciens B1126 strains, which have antagonistic and growth-promoting effects on Trichoderma aureus and Bacillus amyloliquefaciens B1126, were obtained through plate confrontation culture, seedling growth-promoting effect, and seed treatment. Melon crops such as vegetables and cucumbers have a strong induction resistance effect, and the induction resistance effect reaches 50-90%. At the same time, it has a significant growth-promoting effect on the seedling stage and seeds of solanaceous fruits such as tomato, pepper, eggplant, etc. The growth-promoting rate of seedling stage reaches 35-85%, and the rate of promoting seed germination reaches 40-85%.

[0048] Through the wound inoculation safety test, Trichoderma dark green Tr1208 and Bacillus amyloliquefaciens B1126 did not cause wilting or bursting of tomatoes, peppers and tobacco, no lesio...

Embodiment 2

[0050] Identification of Trichoderma dark green.

[0051] Trichoderma strain Tr1208 grows rapidly on the PDA medium, the growth diameter of the colony is greater than 90mm within 3 days, the surface of the mycelium is loose and flat, and the growth is radial; after 3 days of cultivation, it can be seen that the spores are obviously produced, which are light green at first, and then gradually increase. Dark green, concentric ring pattern.

[0052] Microscopic observation showed that the mycelium of Trichoderma strain Tr1208 was colorless, with complicated branches and septations; the conidia had no septum, were spherical, arranged in heads, and were solitary, with obvious and tiny rough protrusions on the cell wall.

[0053] Genomic DNA of Trichoderma strain Tr1208 was used as a template to amplify using Trichoderma translation elongation factor, forward primer: EF1-728F (5'-CATCGAGAAGTTCGAGAAGG-3'), reverse primer: TEF1rev (5'-GCCATCCTTGGAGATACCAGC-3 '). PCR amplification wa...

Embodiment 3

[0055] Identification of Bacillus amyloliquefaciens.

[0056] Bacillus B1126 is cultured on NA medium, its cells are dirty white, translucent, smooth and moist colonies, slightly raised, round and neat colonies; Gram-stained cells are positive, Gram-positive bacteria, rod-shaped; Contact enzyme test positive; oxidase test negative; glucose positive; lactose positive; sucrose positive. Arabinose, mannitol, and xylose can be used to produce acid and gas.

[0057] Genomic DNA of Bacillus B1126 was used as a template and 16S rDNA was used for amplification. Forward primer: P1 (5'-AGAGTTTGATCCTGGCTCAGAACGAACGCT-3') and reverse primer: P6 (5'-TACGGCTACCTTGTTACGACTTCACCCC-3'). The PCR product was sequenced, and the sequencing results were submitted to GenBank, and the sequence was compared in GenBank. It was identified that B1126 belonged to the genus Bacillus, Bacillus amyloliquefaciens ( Bacillus amylolique faciens ).

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Abstract

The invention relates to a Trichoderma atroviride-Bacillus amyloliquefaciens composite microbial agent for preventing and controlling solanaceous vegetable virosis and a preparation method thereof. The Trichoderma atroviride strain is Tr1208, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No. 6828. The Bacillus amyloliquefaciens strain is B1126, is preserved in the China general microbiological culture collection center (CGMCC) and has an accession number of CGMCC No. 6827. The Trichoderma atroviride-Bacillus amyloliquefaciens composite microbial agent is prepared from Trichoderma atroviride Tr1208 and Bacillus amyloliquefaciens B1126 as active components, agriculturally acceptable carriers and a protection agent. The content of the active components is in a range of 5*10<9> to 2*10<10>cfu / g and the content of the microbial protection agent is in a range of 0.1-1%(w / w). The microbial agent can effectively preventing and controlling solanaceous vegetable viroses such as TMV, CMV and TYLCV, can promote plant growth, has high effects, can be used by a simple method, has a low cost, does not pollute the environment and can improve a plant micro-ecological environment.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a trichoderma-bacillus composite microbial bacterial agent for preventing and controlling viral diseases of solanaceous vegetables, a preparation method and application thereof. Background technique [0002] With the acceleration of the adjustment of my country's agricultural structure and the improvement of people's living standards, the demand for diversification of vegetable varieties and annual balanced supply is increasing day by day. The second largest crop for food. According to statistics from the Ministry of Agriculture, in 2009 my country's vegetable planting area accounted for 43% of the world's total, and its total output accounted for 49.6% of the world's total. The total output and export volume both rank first in the world, and it can be called the world's largest vegetable country. [0003] However, with the development of vegetable produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20A01N63/00A01N63/04A01P1/00A01P21/00C12R1/885C12R1/07
Inventor 王勇高苇张春祥王万立郝永娟刘春艳霍建飞
Owner 텐진인스티튜트오브플랜트프로텍션
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