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Method for eliminating bacterial pollution of tissue culture seedlings

A technology for tissue culture and bacterial contamination, applied in the field of eliminating bacterial contamination of tissue culture seedlings, can solve the problems of unsatisfactory sterilization effect, no promotion value, complicated operation, etc., to solve the problem of unsatisfactory sterilization effect and shorten the rehydration time , Good sterilization effect

Inactive Publication Date: 2016-05-11
GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Still another object of the present invention is to provide a method for eliminating bacterial contamination of tissue culture seedlings. In this method, Chinese herbal medicines such as Houttuynia cordata, aloe vera, goose herb, Artemisia argyi, Huoxiang and garlic are added to the plant after reasonable compatibility and treatment. In the conventional culture medium, it is prepared as a decontamination medium, which is used to transfer contaminated tissue culture seedlings. This method has a good bactericidal effect and is easy to operate, and can solve the problems of unsatisfactory sterilizing effect, complicated operation, and easy secondary contamination in the prior art. Sub-pollution and problems with no promotional value

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0031] A method for eliminating bacterial contamination of tissue culture seedlings, comprising the steps of:

[0032] Step A, take by weighing 5 parts by weight of fresh Houttuynia cordata, 5 parts by weight of fresh aloe vera, 15 parts by weight of fresh goose not eating grass, 15 parts by weight of fresh Artemisia argyi, 8 parts by weight of fresh ageratum and 10 parts by weight of Garlic, mixed and beaten to obtain the first Chinese herbal medicine pulp;

[0033] Step B, adding the first Chinese herbal medicine slurry into 100 parts by weight of conventional turmeric medium, mixing and autoclaving to obtain the first decontamination medium;

[0034] Step C, transfer the polluted turmeric tissue culture seedlings to the first decontamination medium, cultivate under the conditions of a culture temperature of 23-27° C., a light intensity of 1500 lux, and a light time of 8-10 h / d, every week After one transfer, after four weeks of cultivation, the pollution can be eliminated ...

Embodiment 2

[0037] A method for eliminating bacterial contamination of tissue culture seedlings, comprising the steps of:

[0038] Step A, take by weighing 8 parts by weight of fresh Houttuynia cordata, 5 parts by weight of fresh aloe vera, 15 parts by weight of fresh goose not eating grass, 10 parts by weight of fresh Artemisia argyi, 8 parts by weight of fresh ageratum and 10 parts by weight of Garlic, mixed and beaten to obtain the first Chinese herbal medicine pulp;

[0039] Step B, adding the first Chinese herbal medicine slurry into 100 parts by weight of the conventional culture medium of C. labialis, mix well and then sterilize by autoclaving to obtain the first decontamination medium;

[0040] Step C. Transfer the polluted tissue-cultured seedlings of Lettuce labiata macula to the first decontamination medium, under the conditions of a culture temperature of 23-27°C, a light intensity of 1500 lux, and a light time of 8-10 h / d Cultivation, transfer once a week, after three weeks ...

Embodiment 3

[0043] A method for eliminating bacterial contamination of tissue culture seedlings, comprising the steps of:

[0044] Step A, take by weighing 10 parts by weight of fresh Houttuynia cordata, 5 parts by weight of fresh aloe vera, 16 parts by weight of fresh goose not eating grass, 15 parts by weight of fresh Artemisia argyi, 5 parts by weight of fresh ageratum and 20 parts by weight of Garlic, mixed and beaten to obtain the first Chinese herbal medicine pulp;

[0045] Step B, adding the first Chinese herbal medicine slurry into 100 parts by weight of conventional Polygonatum culture medium, mixing and autoclaving to obtain the first decontamination medium;

[0046] Step C, transfer the polluted Polygonatum tissue culture seedlings to the first decontamination medium, cultivate them at a culture temperature of 23-27°C, a light intensity of 1500 lux, and a light time of 8-10h / d, every week Transplant once, after four weeks of cultivation, the pollution can be eliminated, and ro...

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Abstract

The invention discloses a method for eliminating bacterial pollution of tissue culture seedlings. The method comprises the following steps: step A, weighing the following raw materials in parts by weight: herba houttuyniae, aloes, small centipeda herb, folium artemisiae argyi, herba pogostemonis and garlic; mixing the raw materials and then pulping or mixing the raw materials, adding water for immersing for a period of time and pulping to obtain Chinese herbal medicine pulp; step B, adding the Chinese herbal medicine pulp into a certain amount of conventional culture medium in parts by weight; uniformly mixing and then carrying out high-pressure sterilization to obtain a pollution removing culture medium; step C, transferring polluted tissue culture seedlings into the pollution removing culture medium and culturing for 7 to 10 days; step D, repeating the step A to the step C until pollution of the polluted tissue culture seedlings is eliminated, so as to obtain pollution-removed culture medium seedlings; step E, transferring the pollution-removed culture medium seedlings into the conventional culture medium and continually culturing. The method disclosed by the invention has the advantages of good sterilization effect, small damages to the culture medium seedlings, low cost, and simplicity and convenience for operation, and is suitable for industrial popularization and the like.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture. More specifically, the present invention relates to a method for eliminating bacterial contamination of tissue culture seedlings. Background technique [0002] In the process of plant tissue culture, pollution is one of the three major problems in the current tissue culture process. Due to various reasons, bacterial contamination of tissue culture seedlings occurs, and the contaminated tissue culture seedlings are generally discarded and used in production. Contaminating bacteria grow rapidly during the process and are easy to spread. If not handled properly, it will cause a devastating blow, increase production costs, and often cause irreparable losses to the preservation of plant resources. At present, in tissue culture, antibiotics are mostly used for sterilizing treatment, but so far no antibiotics have been found to be effective against various bacteria, and the effect is not i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 王晓峰李刚韦坤华肖冬李翠黄宝优缪剑华
Owner GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS
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