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Rapid propagation method for stem segments of Dendrobium huoshanense

A technology for Dendrobium huoshanensis and stem segments, which is applied in the field of rapid propagation of Dendrobium huoshanense stem segments, can solve the problems of low reproduction rate of Dendrobium huoshanense, high prices of Dendrobium huoshanense seedlings, reduction of wild resources, etc., and achieves guaranteed medicinal value and simple and easy reproduction method. The effect of the line, the process is simple

Inactive Publication Date: 2016-05-04
ANHUI HUOSHAN NONGBOLE DEV CO LTD
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AI Technical Summary

Problems solved by technology

In the wild state, Dendrobium Huoshanense has low reproductive rate and slow growth, coupled with predatory excavation, its wild resources are decreasing day by day, which is far from meeting the needs of market economic development and clinical medicine at home and abroad.
Dendrobium seedlings are expensive, resulting in high cultivation costs and low economic benefits. These problems have long plagued Dendrobium growers

Method used

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Experimental program
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Embodiment 1

[0020] A method for rapid propagation of stems of Dendrobium huoshanense, comprising the following steps:

[0021] (1) Preparation of explants

[0022] Select the annual stem section of Dendrobium Huoshanense with good quality, strong growth, obvious stem nodes, and no damage by diseases and insect pests as explants, rinse the explants with running water, and then soak the explants with 75% alcohol on a sterile operating table for 30 seconds, and then sterilized with 0.1% mercuric chloride solution for 15 minutes, then rinsed with sterile water for 5 times, took sterile filter paper to absorb the moisture on the surface of the explant, and cut it into at least one stem node under aseptic conditions. stem segment;

[0023] (2) Induction culture

[0024] Inoculate the stem segments in sterile induction medium, the induction medium formula is: N6+2.5mg / L6-BA+0.75% agar+3% sucrose+8% coconut milk+0.4% activated carbon, the pH value is 5.4~ 5.9; the culture conditions are temper...

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Abstract

The invention discloses a rapid propagation method for stem segments of Dendrobium huoshanense, belonging to the technical field of plant cultivation. The method comprises the following steps: explant preparation, induced culture, enrichment culture, and strong seedling rooting culture. According to the invention, materials are easily available and are not limited by seasons; a stem segment culture manner of bud generation from buds has simple generating process and can well maintain biological characters of female parentd; thus, medicinal values of Dendrobium huoshanense are guaranteed. The propagation method is simple and practicable and solves the technical problems of insufficient explant sterilization in asexual propagation of Dendrobium huoshanense, slow protocorm differentiation, long propagation cycle, high production cost, etc. The rapid propagation method provided by the invention can be applied in actual production, is capable of providing guarantee for planting development of Dendrobium huoshanense, and has good economic benefits and social benefits.

Description

technical field [0001] The invention relates to a method for rapid propagation of stem segments of Dendrobium huoshanense, belonging to the technical field of plant cultivation. Background technique [0002] Huoshan Dendrobium, commonly known as Mi Huo, is a herbaceous plant of the genus Dendrobium orchidaceae. It is a product of China's national geographical indication. It is mainly produced in Huoshan County, Anhui Province in the Dabie Mountains. Grows on tree trunks in mountain forests and on rocks in valleys. Dendrobium Huoshanense can greatly increase the level of SOD (the main substance that delays aging) in the human body. It is very suitable for people who often stay up late, use their brains, smoke and drink excessively, and are physically weak. Dendrobium Huoshanense has the effect of improving eyesight, and can also reconcile yin and yang, strengthen yang and invigorate the kidney, nourish the face and maintain the appearance, so as to achieve the effect of heal...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 万继银万彬
Owner ANHUI HUOSHAN NONGBOLE DEV CO LTD
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