Method for detoxification culture of lily by using acyclovir agent

Abstract

The present invention discloses a method for detoxification culture of lily by using an acyclovir agent, and belongs to the fields of plant tissue culture rapid propagation technologies and biotechnologies. The method comprises: lily seedball virus detection, preparation of culture medium to be used, explant sterilizing and inoculating, explant culture, tissue culture seedling virus detection and other steps so as to rapidly obtain a large number of detoxification tissue culture seedlings. According to the present invention, the optimal induction culture medium formula for direct differentiation of the lily into the bud and the root-free tissue culture seedling is obtained through the orthogonal method, and the acyclovir agents with a series of concentration gradients are prepared to screen the optimized detoxification concentration, wherein the detoxification efficiency can achieve 90%, the induction culture period can be shortened, the characteristics of high detoxification rate, high value-added factor and the like are provided, and the technical foundation is provided for cultivation of lily virus-free plants and promotion of research on non-toxic cultivation.

Description

technical field [0001] The invention discloses a method for detoxifying lilies by using acyclovir agent, which belongs to the field of plant tissue culture rapid propagation technology and biotechnology. Background technique [0002] Lily is the general name of the genus Lilium (Lilium) of the monocot class Liliaceae (Liliaceae), which is a perennial herbaceous flower. There are more than 90 species of Lilium in the world, and about 47 species originate in my country, accounting for 51% of the world total. There are many varieties of lilies, with huge flowers, rich colors, and beautiful flowers. They can be used as cut flowers, potted flowers, and in gardens and green spaces. Most lilies are edible, and they are the best nourishing products. Lily has the meaning of "a hundred years of harmony" and is deeply loved by people. Lilies are mainly distributed in temperate regions such as China, Japan, North America and Europe. In recent years, the production of cut lilies in my ...

AI Technical Summary

Problems solved by technology

The survival rate of shoot tip culture is low