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Preparation method of composite sensitive film electrochemical sensor for detecting epinephrine

A technology of epinephrine and sensitive membrane, which is applied in the field of analytical chemistry and electrochemistry, which can solve the problems of narrow linear range, high detection limit, cumbersome operation, etc., and achieve the effect of enhancing detection sensitivity, excellent electrical conductivity, and enhanced electrical conductivity

Inactive Publication Date: 2016-04-20
UNIV OF JINAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This method has a high detection limit and a narrow linear range, making it difficult to effectively detect trace amounts of adrenaline
Patent No. 200610019708 discloses the preparation of a fiber optic biosensor and a composite sensitive membrane for the detection of adrenaline. The fiber optic biosensor is complicated in equipment and cumbersome in operation, and cannot achieve rapid detection

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  • Preparation method of composite sensitive film electrochemical sensor for detecting epinephrine

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Experimental program
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Embodiment 1

[0022] Preparation of composite sensitive membrane based on graphene: Take 10mL1.0mg·mL -1 Graphene oxide aqueous solution and 10mL1.0mg·mL -1 The acidic carbon nanotube suspension was uniformly mixed and ultrasonically treated for 2 h. Then, 20 mg of sodium borohydride was added, stirred vigorously for 5 min, and then heated to reflux at 130° C. for 3 h. The obtained product was centrifuged and washed with water, and dried at 30°C to obtain the product rGO / CNTs;

[0023] Take 5mg of titanium dioxide nanoparticles and evenly disperse them in 200mL of ultrapure water, sonicate for 10min, add 15mL of 0.1M sodium citrate, stir magnetically and slowly add 0.5mL of 0.1M hydroxylamine hydrochloride solution, then add 1.0mL of 1% chloroauric acid solution, stirred overnight, when the color of the solution turned orange, centrifuged and washed several times with water to obtain TiO 2 - Au nanoclusters;

[0024] Take 0.1grGO / CNTs and 10mgTiO 2 -Au nanoclusters were uniformly disper...

Embodiment 2

[0027] Preparation of composite sensitive membrane based on graphene: Take 20mL1.0mg·mL -1 Graphene oxide aqueous solution and 15mL1.0mg·mL -1 The acidic carbon nanotube suspension was uniformly mixed and ultrasonically treated for 2 h. Then, 30 mg of sodium borohydride was added, stirred vigorously for 5 min, and heated to reflux at 130° C. for 3 h. The obtained product was centrifuged and washed with water, and dried at 30°C to obtain the product rGO / CNTs;

[0028] Take 10mg of titanium dioxide nanoparticles and evenly disperse them in 150mL of ultrapure water, sonicate for 10min, add 20mL of 0.1M sodium citrate, stir magnetically and slowly add 1.0mL of 0.1M hydroxylamine hydrochloride solution, then add 2.0mL of 1% chloroauric acid solution, stirred overnight, when the color of the solution turned orange, centrifuged and washed several times with water to obtain TiO 2 - Au nanoclusters;

[0029] Take 0.15grGO / CNTs and 20mgTiO 2 -Au nanoclusters were uniformly dispersed...

Embodiment 3

[0032] Detection of epinephrine content in urine samples based on graphene composite sensitive membrane electrochemical sensor:

[0033] Modified electrode rGO / CNTs / TiO 2 -Au / GCE is the working electrode, the Ag / AgCl electrode is the reference electrode, and the platinum electrode is the counter electrode; within the potential range of 0.0-0.7V, differential pulse scanning is performed on the adrenaline standard solution samples with different concentrations, according to the peak current value and The relationship between the concentration of epinephrine, draw the working curve, the linear range is 1.0 ~ 300nM, the linear equation is ΔI p =0.5533+0.0313c, c is concentration, unit is nM, peak current I p The unit is μA, and the detection limit is 0.34nM;

[0034] Detection of epinephrine content in urine samples: modified electrode rGO / CNTs / TiO 2 -Au / GCE is the working electrode, the Ag / AgCl electrode is the reference electrode, and the platinum electrode is the counter elect...

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Abstract

The invention discloses a preparation method of a composite sensitive film electrochemical sensor for detecting epinephrine. The method takes graphene as a carrier, and an acidic carbon nano tube is covalently combined with the surface of the carrier, so that an electron transferring performance is enhanced; a titanium dioxide nano cluster, which is loaded with nano-gold on the surface, is loaded on the surface of a graphene / carbon nano tube covalent matter, so that a reaction site and reaction activity are improved. The surface of a glassy carbon elctrode is modified with a sensitive film based on the graphene to construct the electrochemical sensor for detecting the epinephrine. The electrochemical sensor constructed by the sensitive film based on the graphene has ultrahigh sensitivity on detection of the epinephrine, a wide detection range and extremely low detection limit, and rapid and sensitive detection on an actual urine sample can be realized.

Description

technical field [0001] The invention relates to the preparation of a composite sensitive membrane electrochemical sensor for detecting adrenaline, which belongs to the technical fields of electrochemistry and analytical chemistry. Background technique [0002] Adrenaline is the main hormone of the adrenal medulla, which can be secreted when experiencing excitement, tension, fear and other stimuli. Adrenaline can strengthen myocardial contraction, increase excitability, accelerate conduction, and increase cardiac output. Its effect on blood vessels in various parts of the body is not only different in strength, but also in contraction or relaxation. It maintains the internal dynamic balance of the human body and responds to acute and chronic stress through cardiovascular mediation, metabolism, and visceral movement. On the one hand, it can constrict the blood vessels of the heart, strengthen the activity of the heart and brain, and increase the blood pressure sharply; capa...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/3277G01N27/3278
Inventor 李剑波罗川南王晓娇段辉敏王延辉
Owner UNIV OF JINAN
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