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Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof

A technology for bacillus and soft rot, applied in the field of microorganisms, can solve problems such as increasing the residual amount of toxic chemicals, polluting the ecological environment, and drug resistance of pathogenic bacteria, achieving good control effects, huge application potential, and convenient operation

Inactive Publication Date: 2016-04-20
北京市辐射中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Every year in my country, the pear ring disease damages the production of pear fruit by about 25%, and the fruit rot rate is as high as 80% in severe cases, causing huge economic losses.
At present, chemical agents are often used in production to prevent and control postharvest diseases of pears, which not only seriously pollutes the ecological environment, but also increases the residual amount of toxic chemicals in agricultural products, which brings potential harm to human health and also easily induces the emergence of drug resistance of pathogenic bacteria

Method used

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  • Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof
  • Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof
  • Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. Isolation and identification of Bacillus siamese strains

[0041] 1. Isolation and purification of strains

[0042] 1. Rinse Hongxiao pears from Beijing, Laiyang pears from Shandong, Xuehua pears from Hebei, and Dangshan pears from Anhui with tap water, rinse with distilled water, peel off the peels after drying, and disinfect the peels. The disinfection method is as follows: 70% alcohol treatment for 40s, rinsed with sterile water, then treated with 2.5% sodium hypochlorite solution for 2min, rinsed 3-4 times with sterile water.

[0043] 2. Under aseptic conditions, take 100 μL of sterile water produced by the last rinse and spread it on the beef extract medium, incubate at 30°C for 24-72 hours, and observe the formation of no colonies. Verify whether this disinfection method kills or not. microorganisms on the surface of the test material.

[0044] 3. In aseptic state, cut the peel into 0.2cm 2 The fragments were placed in plates containing beef extract ...

Embodiment 2

[0062] Embodiment 2, the application of bacterial strain LYLB4 in antibacterial or antibacterial

[0063] 1. Antagonistic activity of strain LYLB4 against Pear rot and soft rot

[0064] The pathogenic strains Botryosphaeria dothidea and Rhizopus stolonifer were respectively activated on PDA medium and punched with a 6 mm hole punch to obtain Botryosphaeria dothidea and Rhizopus stolonifer respectively. Using the confrontation culture method, respectively inoculate the pear rot disease cake and soft rot bacteria cake on one side of a PDA plate with a diameter of 9 cm, and streak the opposite side to inoculate the strain LYLB4, and the two inoculation points are separated by 3-4 cm. The pathogenic strains of Verticillium pear and Pseudomonas soft rot on the PDA plate were used as controls. Three replicates were set up and cultivated at 25°C. When the control was covered with the whole dish, the diameter of the treated colony and the width of the inhibition zone were measured, a...

Embodiment 3

[0077] Example 3. Application of strain LYLB4 in the prevention and treatment of pear fruit ring disease

[0078] 1. The control effect of strain LYLB4 on pear fruit ring disease

[0079] 1. Pear pretreatment

[0080] Select fruits with neat appearance, no pests and diseases, and no trauma, rinse them with tap water first, then spray and disinfect with 75% alcohol, and dry the surface with sterile filter paper after 2 minutes for use.

[0081] 2. LYLB4 bacterial suspension treatment

[0082] Two wounds with the same depth as possible were symmetrically punched on the fruit surface with a sterile hole punch (diameter 6mm), and 50 μL of 5×10 wounds were inoculated into the wounds. 7 CFU / ml of LYLB4 bacterial suspension (LYLB4 treatment group) and the same amount of sterile water (control group), 8 fruits per treatment, repeated 3 times, and placed at room temperature for one day.

[0083] 3. Inoculation with pathogenic bacteria

[0084]The wounds of the LYLB4 treatment group...

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Abstract

The invention discloses a Bacillus siamensis strain for preventing and treating pear ring rot and soft rot and application thereof. The Bacillus siamensis strain is separated from peels of Laiyang pears with sterilized surfaces, the strain has broad-spectrum resistance for various types of phytopathogens and has a good effect on preventing and treating the pear fruit rot caused by pear ring rot and soft rot. The strain comes from pear fruits and is safe to pear fruits; the strain is produced on a large scale, is low in raw material cost, convenient to operate and stable in prevention and treatment effect, and can effectively solve the problems of agricultural residues and environment pollutions caused by pear fruit disease and insect pesticide prevention and treatment. The strain has huge application potential on the aspects of biological prevention and treatment of after-harvest diseases and insects of pears, and the excellent basic strain is provided for research and development of microbial pesticides.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a bacillus siamese strain for preventing and treating pear ring spot and soft rot and application thereof. Background technique [0002] Pear is one of the fruit trees with a long history of cultivation, large area and high yield in my country. According to statistics, pear is the third largest fruit in my country. At present, the total area of ​​the country is more than 1 million hectares, and the annual output is more than 8 million tons, accounting for 62% and 52% of the world's pear cultivation area and output respectively. Pear fruit is threatened by diseases all the time during growth and storage, among which Botryosphaeria dothidea is one of the most serious fruit fungal diseases and is the main cause of pear fruit rot. Because the ringworm is a latent infection in the young fruit stage, it will develop during the ripening and storage of the fruit, so it is very ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A23B7/155C12R1/07
CPCA01N63/00A23B7/155C12N1/205C12R2001/07
Inventor 武利勤尚宏忠顾海科王青
Owner 北京市辐射中心
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