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A kind of agarose crystal gel matrix particles with super large pores and preparation method thereof

A technology of matrix particles and agarose, which is applied in the field of agarose crystal gel matrix particles and their preparation, can solve problems such as lack of research and development, and achieve the effects of good permeability performance, rapid mass transfer and simple preparation method.

Active Publication Date: 2019-07-23
ZHEJIANG UNIV OF TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is little research on agarose matrix and its separation medium with a pore size of several microns to hundreds of microns, especially the research and development of large-sized crystal gel matrix particles with a particle size of more than 1 mm and its preparation methods are very scarce.

Method used

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  • A kind of agarose crystal gel matrix particles with super large pores and preparation method thereof
  • A kind of agarose crystal gel matrix particles with super large pores and preparation method thereof
  • A kind of agarose crystal gel matrix particles with super large pores and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Dissolve 3 g of agarose in 97 mL of deionized water at 75-95 °C to make a 3% agarose solution; then, pass the obtained agarose solution through a multi-microtube reactor (microtube inner diameter 0.5 cm) at a flow rate of 5 cm / s. mm) to form droplets, freeze and crystallize in an ethyl acetate solution containing 3% Span 80 surfactant to form pores, and melt the ice crystals at room temperature to obtain a super-porous agarose crystal gel matrix. The actual morphology is as follows: figure 2 As shown, its cross-sectional enlarged structure is as follows figure 1 As shown, after measurement, the super-porous agarose crystal gel matrix has an average particle size of about 2 mm and a porosity of 97%. It is a submicron small pore 3, and the skeleton is surrounded by a large pore 2. As shown in the figure, the pore size of the super pore is 5~200 μm, and it has good elasticity in water.

Embodiment 2

[0028] Dissolve 6 g of agarose in 94 mL of deionized water at 75-95 °C to make a 6% agarose solution; then, pass the obtained agarose solution through a multi-microtube reactor (microtube inner diameter 2.2 mm) at a flow rate of 0.9 cm / s. mm) to form droplets, freeze and crystallize in ethyl heptanoate solution containing 2% Span 80 to form pores, and melt the ice crystals at room temperature to obtain a super-porous agarose crystal gel matrix. It is determined that the average particle size is about 4.3 mm, the porosity is 94%, and the colloidal matrix skeleton accounts for 6% of the total volume of the particles. There are submicron small pores in the skeleton, and the pore size of the large pores around the skeleton is 5~150 μm, which has good elasticity in water. .

Embodiment 3

[0030] Dissolve 6 g of agarose in 194 mL of deionized water at 75-95 °C to make a 3% agarose solution; then, pass the obtained agarose solution through a multi-microtube reactor (microtube inner diameter) at a flow rate of 4.2 cm / s. 2.2 mm) to form droplets, freeze and crystallize in a cyclohexane solution containing 80% by mass of Span to form pores, and melt the ice crystals at room temperature to obtain a super-porous agarose crystal gel matrix. The agarose granular crystal gel The schematic diagram of the structural cross-section of the matrix is ​​as follows: figure 1 As shown, its actual shape is as follows figure 2 shown. The average particle size was determined to be about 5 mm, the porosity was 97%, and the colloidal matrix skeleton accounted for 3% of the total particle volume. The skeleton contained submicron small pores, and the large pores around the skeleton had a pore size of 5-200 μm, which had good elasticity in water.

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Abstract

The invention relates to an agarose crystal gel matrix particle with extra large pores and a preparation method thereof. The agarose crystal gel matrix particle is characterized in that the super-pore agarose crystal gel matrix particle is a porous particle, and has the super pore of tens to hundreds of microns, the particle size of 2-5mm, the pore diameter of 5-200 micrometers and the porosity of 94-97 percent. The invention also provides a method for preparing the agarose crystal gel matrix particle by multi-micropipe reactor droplet forming and solvent crystallization hole forming. The agarose crystal gel matrix particle has good biocompatibility and the mutually communicated extra large pores, is high in porosity, excellent in permeability, and easy for realizing in-pore convective mass transfer of target objects, and therefore can serve as base materials of carriers for immobilization and biological separation media. The preparation method is simple, parallel amplification can be realized, large-scale industrial preparation is convenient, and the agarose crystal gel matrix particle with the extra large pores and the preparation method thereof have wide application prospect in the fields of biological separation, tissue engineering, immobilization of enzymes or cells, and the like.

Description

technical field [0001] The invention belongs to the technical field of biochemical industry and separation, and in particular relates to agarose crystal gel matrix particles with super-large pores and a preparation method thereof. Background technique [0002] Crystal colloid medium is a new type of biological separation medium with super-large pores ranging from several microns to hundreds of microns, and it is also a new type of super-porous elastic porous material. Crystal gel can not only be used as a separation filler and has broad application prospects in the field of biological separation, but also can be used as a new carrier for tissue engineering scaffold carrier, microbial cell embedding and immobilization enzyme carrier. The skeleton materials of the existing crystal glue are mainly synthetic high molecular polymers such as polyacrylamide, polyvinyl alcohol, polyhydroxyethyl methacrylate, etc., while the research and development of the crystal glue medium of natu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J9/28C08J9/00C08L5/12
CPCC08J9/00C08J9/28C08J2201/0484C08J2305/12
Inventor 贠军贤楼小玲贠玉龙江涛
Owner ZHEJIANG UNIV OF TECH
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