Induction and propagating method of Tetrastigma hemsleyanum Diels et Gilg adventitious root

A cloverleaf and induction medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve problems such as bottlenecks in the production of gourd tuberous roots and tuberous roots, and achieve the effect of solving the problem of contradiction between supply and demand

Active Publication Date: 2016-04-20
HANGZHOU ACAD OF AGRI SCI
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Problems solved by technology

[0004] At present, the technical problems of domestication cultivation, in vitro cutting and tissue culture and rapid propagation of Clover have been basically solved, but there are serious bottlenecks in the production technology of its artificially cultivated Chinese medicinal parts gourd tubers and tubers.

Method used

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Embodiment Construction

[0019] The preferred embodiments of the present invention will be described in detail below.

[0020] The present embodiment trefoil adventitious root induction and propagation method, it carries out as follows:

[0021] (1) Induction of callus: rinse the leaves extracted from the Clover plant, sterilize with ethanol on the ultra-clean workbench and transfer to mercuric chloride for disinfection, then rinse with sterile water for 5 to 6 times, Cut into about 0.5cm×0.5cm in size, put it face up on the induction medium, and culture in the dark at 27±1°C for 30 days. In this step, the concentration of ethanol is 75%, soak the leaves for 30 seconds, transfer to 0.1% mercuric chloride for immersion and disinfection for 10 minutes, and the induction medium formula is MS+30g L -1 Sucrose+2.0mg·L -1 6-BA+0.2mg·L -1 NAA+6.5g·L -1 agar.

[0022] (2) Proliferation of callus: Cut the callus obtained in step (1) into small pieces on an ultra-clean workbench, inoculate on the prolifera...

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Abstract

The invention discloses an induction and propagating method of a Tetrastigma hemsleyanum Diels et Gilg adventitious root, comprising the following steps of (1) induction of callus: cleaning leaves picked from a Tetrastigma hemsleyanum Diels et Gilg plant, sterilizing the leaves with ethyl alcohol on an ultra-clean workbench, then sterilizing the leaves with mercury bichloride, washing with sterile water, cutting, horizontally placing on an inducing medium with the right sides of the leaves being upward, and performing dark culture at 27+ / -1 DEG C; (2) propagation of the callus: cutting the callus obtained in step (1) into small pieces on the ultra-clean workbench, inoculating the cut callus with a propagation medium, and performing dark culture at 27+ / -1 DEG C; (3) induction of callus differentiation adventitious root: cutting the callus obtained in step (2) into small pieces on the ultra-clean workbench, inoculating the cut callus with an adventitious root induction medium, and performing dark culture at 27+ / -1 DEG C; (4) propagation of the adventitious root: cutting the adventitious root obtained in the step (3) into the set length on the ultra-clean workbench, inoculating the cut adventitious root with a liquid culture medium of propagation of the adventitious root, performing dark culture, and then performing light culture.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for inducing and propagating adventitious roots of P. trifoliate. Background technique [0002] Cliff climbing vine (Tetrastlg mahemsleyanum Dielset Gilg) is a unique rare medicinal plant in China. It is mainly used as medicine with tubers. It is cool in nature, non-toxic, sweet and slightly bitter. It has a remarkable curative effect on viral meningitis, rheumatoid arthritis, gynecological diseases, blood metrorrhagia, leucorrhea, blood diseases, cardiovascular and cerebrovascular diseases, poisonous snake bites, carbuncle, anal fissure, anti-HIV and anti-tumor clinical treatment It has a good curative effect and is called "plant penicillin". It has been found that the flavonoid extract of clover has effects on H-22, SMMC-7721 and HepG2 liver cancer cells, A549 lung cancer cells, SGC-7901 human gastric cancer cells, colon cancer cell lines RKO cells and HT-29 c...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/005
Inventor 钱丽华马华升姜慧燕张乐王贤波刘洋
Owner HANGZHOU ACAD OF AGRI SCI
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