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Applications of calcium-and inte-grin-binding protein 1 in preventing and treating enterovirus 71 infection

A technology that binds proteins, enteroviruses

Active Publication Date: 2016-03-30
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Abstract
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  • Application Information

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Problems solved by technology

[0007] At present, there is no research report on the role of CIB1 molecule in EV71 infection of human neuroblastoma (SH-SY5Y). In-depth research on CIB1 molecule can not only improve the understanding of EV71 infection and pathogenic mechanism, but also contribute to the prevention and treatment of human neuroblastoma. Treatment of EV71 infection provides new ideas and targets

Method used

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  • Applications of calcium-and inte-grin-binding protein 1 in preventing and treating enterovirus 71 infection
  • Applications of calcium-and inte-grin-binding protein 1 in preventing and treating enterovirus 71 infection
  • Applications of calcium-and inte-grin-binding protein 1 in preventing and treating enterovirus 71 infection

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Embodiment 1

[0048] 1 Design and synthesis of specific siRNA sequences for each host cell molecule.

[0049] 1.1 For each target gene, search NCBI GeneBank to obtain the full sequence and mRNA sequence, use existing network resources and commonly used software to conduct biological analysis of each target gene, and select the coding region as the target sequence for siRNA design. Refer to the siRNA design principle, and compare it with the human genome sequence through the blast function of the GeneBank database to ensure that there is no homology; exclude potential siRNAs that have 8 consecutive bases at the 5' end of the aitisense chain paired with other genes; exclude any consecutive 14 bases base-pairing potential siRNAs with other genes. The design software was used for pre-evaluation and determination, and three targets with the best kinetic parameters were selected to enter the subsequent experimental process. A total of three interference sequences were synthesized for each gene, a...

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Abstract

The invention belongs to the technical field of biomedicine, and relates to a novel target used for preventing enterovirus 71 infection, and applications thereof. According to the invention, SH-SY5Y is taken as the target cell, and RNA interference technology is adopted for down-regulation of expression of target cell host protein so as to search host factors capable of inhibiting EV71 infection of SH-SY5Y cells effectively and protect the functions of central nervous systems. It is fond by experiments that calcium-and inte-grin-binding protein, CIB1 molecular possesses significant importance in EV71 infection of SH-SY5Y, and down-regulation of expression of CIB1 is capable of inhibiting EV71 infection obviously. The invention also provides applications of CIB1 in preparing drugs used for preventing or treating EV71 infection.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a new target and application of anti-enterovirus 71 infection. Background technique [0002] Enterovirus 71 (enterovirus71, EV71) belongs to the Picornaviridae Enterovirus genus Human Enterovirus A, and is one of the main pathogens that cause Hand-foot-mouth disease (HFMD). Currently, hand, foot and mouth disease is breaking out and spreading in many parts of the world, especially in the Asia-Pacific region. In my country, since the outbreak of hand, foot and mouth disease in several major provinces and cities in 2008, the number of people infected and the death rate of the disease have remained high, with more than 1 million cases and nearly 1,000 deaths reported every year. The main patients with HFMD are infants and young children under 5 years old. The clinical manifestations are fever, herpes and other symptoms in the hands, feet, buttocks and oral mucosa; a small numbe...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61P31/14
CPCA61K48/00
Inventor 朱勇喆朱耐伟徐庆强彭浩然戚中田宋洪元
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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