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NMR virus rapid detection method based on KMnF4 nanoprobes

A technology of nano-probe and detection method, which is applied in the field of rapid detection of viruses, and can solve problems such as inapplicability

Inactive Publication Date: 2016-03-23
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Applicable to viruses that can be separated by double-antibody sandwich technology, but not applicable to some special viruses that cannot be separated by double-antibody sandwich technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The sample is tested to determine whether it contains the virus - Bovine Viral Diarrhea Virus.

[0023] 1. KMnF 4 Nanoparticle preparation: KMnF 4 Nanoparticles can be purchased from the market, and can also be prepared by the following method: Weigh 1.6mmolMnCl 2 4H2O was added to the reaction kettle, then 10mL of absolute ethanol was added, and the solution was stirred until it was completely dissolved; 3 mmol of potassium oleate was weighed and added to the above solution, and stirred for 1-2min to mix well; 5-6g of fluoride Potassium (KF) is ground for more than 10 minutes. When the particle size becomes smaller and finer, weigh 4.98mmol and add it to the previous solution; add absolute ethanol again, usually 30mL; Put the kettle into the oven, adjust the temperature to 160°C, and react for 24 hours; when the reaction kettle drops to room temperature, it usually takes 3-4 hours after closing the oven. Open the reaction kettle, pour out the supernatant, wash with ...

Embodiment 2

[0031] Determination of whether the sample contains the virus - porcine parvovirus.

[0032] 1. KMnF 4 Nanoparticle preparation: KMnF 4 Nanoparticles can be purchased from the market, and can also be prepared by the following method: Weigh 1.6mmolMnCl 2 4H2O was added to the reaction kettle, then 10mL of absolute ethanol was added, and the solution was stirred until it was completely dissolved; 3 mmol of potassium oleate was weighed and added to the above solution, and stirred for 1-2min to mix well; 5-6g of fluoride Potassium (KF) is ground for more than 10 minutes. When the particle size becomes smaller and finer, weigh 4.98mmol and add it to the previous solution; add absolute ethanol again, usually 30mL; Put the kettle into the oven, adjust the temperature to 160°C, and react for 24 hours; when the reaction kettle drops to room temperature, it usually takes 3-4 hours after closing the oven. Open the reaction kettle, pour out the supernatant, wash with absolute ethanol a...

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PUM

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Abstract

The invention discloses an NMR virus rapid detection method based on KMnF4 nanoprobes, and belongs to the technical field of food safety rapid detection. The method relies on an established nuclear magnetic resonance detection method capable of being applied to viruses in samples, and whether the samples contain viruses or not is detected by utilizing the influences of the paramagnetic property of the KMnF4 nanoprobes on nuclear magnetic resonance relaxation time. According to different specific corresponding relations, the KMnF4 nanoprobes show a linear relation under certain conditions, namely, the larger the content of the KMnF4 nanoprobes is, the smaller the spin-lattice relaxation time value of the samples will be, and therefore viruses can be quantitatively detected within a certain range. The method can be applied to rapid detection of viruses in the samples, and therefore the method can be applied to rapid screening of a large batch of samples to be detected.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of food safety and relates to a rapid detection method for viruses. Background technique [0002] Basic principle: Monoclonal antibodies or antigen molecules are combined with enzyme molecules through covalent bonds. This combination will not change the immunological characteristics and biological activities of monoclonal antibodies, antigens and enzymes. Specific monoclonal antibodies will only interact with specificity. antigen binding. QUR 4 The nanoprobe is antibody-modified KMnF 4 Nanoparticles, KMnF 4 Due to the element Mn, there are unpaired electrons in the outermost layer, so it has a permanent electron spin. The total magnetic moment of the unpaired electron spin is 657 times that of the proton spin. Since the relaxation efficiency varies with the square of the magnetic moment, the electron relaxation efficiency is 500,000 times that of the proton relaxation efficiency, so i...

Claims

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Application Information

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IPC IPC(8): G01N33/577
CPCG01N33/56983G01N33/54366
Inventor 张锦胜万益琴彭红刘玉环王允圃巫小丹
Owner NANCHANG UNIV
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