Preparation method of porous scaffold material for tissue engineering

Abstract

The invention relates to a preparation method of a porous scaffold material for tissue engineering, which belongs to the field of medical material preparation. The method is implemented by taking sodium alginate with good biocompatibility and lotus fibers as raw materials through the steps of oxidizing sodium alginate, nanocrystallizing lotus fibers, crosslinking the obtained object by using carboxymethyl chitosan, and carrying out vacuum freeze-drying on the obtained product, so that the porous scaffold material is obtained; and according to the invention, the defect that polymeric biomaterials are poor in biocompatibility is overcome, and the shortcomings that pure sodium alginate as a tissue material is poor in mechanical property and slowly degraded are made up. The porous scaffold material prepared according to the invention has good biodegradability, the porous scaffold material is degraded in the process of cell growth and reproduction, and degradation products are non-toxic and harmless; and the porous scaffold material has high porosity and specific surface area, so that the porous scaffold material is applicable to the focal adhesion and growth of cells, the intercellular signal transduction and nutrient transfer, and the discharge of degradation products and metabolic products.

Description

technical field [0001] The invention relates to a preparation method of a tissue engineering porous support material, which belongs to the field of medical material preparation. Background technique [0002] The failure or loss of tissues and organs is a major problem facing human health, and the construction of tissue engineering porous scaffold materials is the core issue to help repair damaged tissues and organs, and has become a hot topic in tissue engineering research. There are many biomaterials used for tissue engineering. Selecting biomaterials with excellent performance is the prerequisite for constructing porous scaffold materials for tissue engineering. Designing a reasonable porous structure and regulating the degradation rate is the key to the completion of the temporary scaffold task. It can be seen that regulation Material structure and properties are the focus of constructing ideal porous scaffold materials. [0003] Cells in different organs have different ...

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: aiming at the current tissue engineering materials mainly composed of synthetic polymer biomaterials, which have excellent mechanical properties but have the defects of poor biocompatibility and low cell affinity, when used for human tissue transplantation, it will cause The generation of rejection reaction provides a preparation method of tissue engineering materials with good biocompatibility, non-toxicity and self-biodegradation. The method uses sodium alginate and lotus fiber with good biocompatibility as raw materials, Sodium alginate was oxidized to make lotus fiber nano, cross-linked with hydroxymethyl chitosan, and vacuum freeze-dried to make a porous scaffold material. As a tissue material, sodium bicarbonate has poor mechanical properties and slow degradation.