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Extraction process of polysaccharide in poria cocos

An extraction process, the technology of Poria cocos, applied in the field of polysaccharide extraction technology, can solve the problems of cumbersome extraction procedures, low polysaccharide extraction rate, and easy damage to the three-dimensional structure of polysaccharides, and meet the requirements of large-scale industrial production, high polysaccharide extraction rate, and process Effects in simple steps

Inactive Publication Date: 2016-03-23
NINGGUO THOUSAND SQUARE TCM DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The water extraction and alcohol precipitation method takes a long time and the extraction rate of polysaccharides is low; the leaching procedure of pachyrhin with acid and alkali solution is cumbersome and the leaching reaction is more violent, which easily destroys the three-dimensional structure of polysaccharides and limits its biological activity.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The extraction technique of polysaccharide in the present embodiment Poria cocos, comprises the following steps:

[0022] (1) Pretreatment: select Poria cocos of good quality, wash and dry, and degrease with anhydrous ether in Soxhlet at 50° C. for 3 hours to obtain fat-free Poria cocos powder;

[0023] (2) Extraction Add distilled water to the Poria cocos powder described in step (1), the ratio of solid to liquid is 1:15, ultrasonically extract at 80°C for 25 minutes, and the ultrasonic power is 240w;

[0024] (3) Suction filtration uses vacuum filtration device to extract the moisture of the solution extracted in step (2);

[0025] (4) decolorization Add gac to the Poria cocos after suction filtration in step (3) and carry out decolorization, obtain the Poria cocos after decolorization;

[0026] (5) concentrating and pouring the decolorized Poria cocos in step (4) into an evaporating dish for concentration to obtain the crude polysaccharide of Poria cocos;

[0027] ...

Embodiment 2

[0030] The extraction technique of polysaccharide in the present embodiment Poria cocos, comprises the following steps:

[0031] (1) Pretreatment: select Poria cocos of good quality, wash and dry, and degrease with anhydrous ether in Soxhlet at 50° C. for 3 hours to obtain fat-free Poria cocos powder;

[0032] (2) Extraction Add distilled water to the Poria cocos powder described in step (1), the ratio of solid to liquid is 1:20, and conduct ultrasonic extraction at 60°C for 35 minutes, with ultrasonic power of 200w;

[0033] (3) Suction filtration uses vacuum filtration device to extract the moisture of the solution extracted in step (2);

[0034] (4) decolorization Add gac to the Poria cocos after suction filtration in step (3) and carry out decolorization, obtain the Poria cocos after decolorization;

[0035] (5) concentrating and pouring the decolorized Poria cocos in step (4) into an evaporating dish for concentration to obtain the crude polysaccharide of Poria cocos;

...

Embodiment 3

[0039] The extraction technique of polysaccharide in the present embodiment Poria cocos, comprises the following steps:

[0040] (1) Pretreatment: select Poria cocos of good quality, wash and dry, and degrease with anhydrous ether in Soxhlet at 50° C. for 3 hours to obtain fat-free Poria cocos powder;

[0041] (2) Extraction Add distilled water to the Poria cocos powder described in step (1), the ratio of solid to liquid is 1:25, ultrasonically extract at 70°C for 30 minutes, and the ultrasonic power is 280w;

[0042] (3) Suction filtration uses vacuum filtration device to extract the moisture of the solution extracted in step (2);

[0043] (4) decolorization Add gac to the Poria cocos after suction filtration in step (3) and carry out decolorization, obtain the Poria cocos after decolorization;

[0044] (5) concentrating and pouring the decolorized Poria cocos in step (4) into an evaporating dish for concentration to obtain the crude polysaccharide of Poria cocos;

[0045] ...

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Abstract

The invention relates to an extraction process of traditional Chinese medicine, in particular to an extraction process of polysaccharide in poria cocos. The extraction process of polysaccharide in poria cocos is low in time consumption and high in extraction rate. The extraction process includes the process steps of performing pretreatment; performing extraction, wherein distilled water is added into degreased poria cocos powder at the material-liquid ratio of 1:15-1:25, and ultrasonic extraction is performed for 25-30 min at 60-80 DEG C at the ultrasonic power of 200-280 w; performing suction filtration, decoloration and concentration; performing deproteinization, wherein crude polysaccharide is dissolved in double-distilled water, the mixture is added into mixed liquor of chloroform and n-butyl alcohol at the mass ratio of 4:1-5:1 after even mixing, the ratio of m chloroform to m n-butyl alcohol in the mixed liquor is 5:1, supernatant liquor is taken after shaking, 3-5-volume fold ethyl alcohol with the mass fraction of 95% is added, the mixture is placed in a refrigerator for 24 h of standing, and then deproteinized poria cocos polysaccharide is obtained through centrifugation; performing washing to obtain poria cocos polysaccharide.

Description

technical field [0001] The present invention relates to a polysaccharide extraction process, more specifically, relates to the extraction process of polysaccharides in poria cocos. Background technique [0002] Poria cocos, English name: Indian Buead, Tuckahoe, Latin name: Wolfiporia cocos (Schw.) Ryv.&Gibn, alias: Poria cocos, Poria spirit, Poria cocos, Fu Ling, Fu doctor, Song Yu, Yun Ling, Poria rabbit, loose potato, Pine cassava, pine Ling. It belongs to the order Aphyllophorales, Polyporaceae, and Wolfiporia. [0003] The dried sclerotia of Poria cocos, its main active ingredient is polysaccharides, mainly pachyman, with a content of up to 75%, which is a kind of polysaccharide with β (1→6) glucopyranose branched chain. β(1→3) glucopyranose, cut the branched chain into β(1→3) glucopyran, called pachymaran, often called pachymaran (PPS), has antitumor activity, inhibits It has various functions such as tumor growth, anti-inflammation, anti-aging, regulation of body im...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 包勇
Owner NINGGUO THOUSAND SQUARE TCM DEV
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