Fluorescent quantitation PCR (Polymerase Chain Reaction) detection kit for human papilloma viruses and application method of fluorescent quantitation PCR detection kit

Abstract

The invention provides a fluorescent quantitation PCR (Polymerase Chain Reaction) detection kit for human papilloma viruses. The detection kit comprises a PCR reaction solution, wherein the PCR reaction solution comprises a PCR buffering solution, deoxyribonucleoside triphosphate, upstream and downstream primers used for amplifying target polynucleotide, and a probe for detecting the target polynucleotide. The fluorescent quantitation PCR detection kit for the human papilloma viruses, provided by the invention, has very good specificity and has the advantages that the operation is rapid, the method is simple and convenient and the sensitivity is high; meanwhile, an interior label, which is increased in a reaction system, can be used for effectively preventing detection from being false negative. The fluorescent quantitation PCR detection kit for the human papilloma viruses, provided by the invention, adopts a rapid nucleic acid releasing method when DNAs (Deoxyribose Nucleic Acid) are extracted; the method is simple, convenient and rapid, and heating is not needed.

Description

technical field [0001] The invention relates to the technical field of medical detection, and more specifically relates to a human papillomavirus fluorescent quantitative PCR detection kit and a use method thereof. Background technique [0002] Human papillomavirus (Humanpapillomavirus, HPV) is a small molecular weight non-enveloped double-stranded circular DNA virus, which obligately infects and parasitizes the epithelial cells of human reproductive organs and other tissues and organs. Clinically, HPV can be divided into high-risk and low-risk types according to the different pathogenicity or cancer risk of different HPV subtypes. Low-risk HPV mainly causes exophytic wart-like lesions on the anus skin, male external genitalia, female labia, urethral meatus, lower vagina, and low-grade cervical intraepithelial neoplasia. High-risk HPV can not only cause external genital warts, but more importantly, external genital cancer, cervical cancer and high-grade cervical intraepithe...

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a human papillomavirus fluorescence quantitative PCR detection kit and its use method, to solve the problem of low sensitivity of the existing HPV detection method described in the background technology

Images