Breeding method for solving color difference problem of colored fresh-eating corn grains
A technology of corn kernels and fresh food, which is applied in the direction of botany equipment and methods, application, plant genetic improvement, etc., can solve the problems of inconsistent shades, large deviation between the ratio of colored kernels and the theoretical value, and impure kernel color during the fresh eating period. To achieve the effects of short breeding cycle, fast homozygosity and low cost
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Embodiment 1
[0051] Example 1. Color purification technology of 'Shen Nong Hong Nuo' fresh waxy corn
[0052] 'Shennong Hongnuo' is a pink fresh waxy corn hybrid, which belongs to the aleurone-type colored corn. Due to the genetic complexity of the gene controlling the red aleurone layer, this variety exhibited inconsistent color at the harvest stage of fresh ears. Therefore need to purify color, reach the pure and even purpose of the seed color of milk stage.
[0053] (1) Homozygous genotypes of the two inbred lines
[0054] Using the conventional colchicine induction method, the genotypes of the female inbred line 'Hongnuo 80' and the paternal inbred line 'Hongnuo 68' were homozygous: inject colchicine inducer into the silk at the silking stage, Chromosome doubling of the female gamete directly forms diploid seeds. Seeds with different genotypes, ie different doses of color genes, are obtained after maturity. The number of induced strains was 50.
[0055] (2) Color identificat...
Embodiment 2
[0061] Example 2. Color purification technology of 'Kennian No. 1' yellow glutinous fresh corn
[0062] 'Kennian 1' is a variety of yellow-grained fresh waxy corn, but the color of the endosperm shows different shades among different kernels of the same ear. Therefore, it is necessary to purify the genotype to make the grain color uniform.
[0063] (1) Homozygous genotypes of the two inbred lines
[0064] Using the existing trifluralin herbicide induction technology, the genotypes of the female parent inbred line 'Nuo 1' and the paternal inbred line 'Nuo 2' were homozygous: Inject trifluralin inducer into the silk during the silking stage , so that the chromosomes of the female gamete directly double to form diploid seeds without double fertilization. Seeds with different genotypes, ie different doses of color genes, are obtained after maturity. The number of induced strains was 50.
[0065] (2) Color identification and screening of double inbred lines
[0066] Duri...
Embodiment 3
[0071] Example 3. Color purification technology of 'Jinghuanuo 2008' Huanuo fresh corn
[0072] ‘Jinghua Nuo 2008’ is a single-cross variety of red and white colored fresh edible waxy corn, which belongs to the aleurone-type colored corn. This variety is a hybrid of two inbred lines, Bainuo 'N203' and Hongnuo 'ZN6'. In theory, F1 grain color should be white: red = 1:3. But in fact, the grain color of this variety is white, pink, and grains with different degrees of pink halo in the fresh ear harvest period, and there are fewer colored grains and more white grains, and the white grains exceed 1 / 4 of the theory Value, darker kernels are less than 1 / 4 of the theoretical value. It is necessary to purify the genotype so that the color of the late stage grains will be darker, and the proportion of colored grains will reach the theoretical value.
[0073] (1) Purification of the genotypes of the parental inbred lines
[0074] Using the conventional colchicine induction method...
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