A KMnF4 nanoprobe-based NMR rapid detection method for food-borne allergens

A food-borne allergen, nano-probe technology, applied in measurement devices, material analysis through resonance, instruments, etc., can solve the problem of no cure for allergic diseases.

Inactive Publication Date: 2016-02-24
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no cure for some allergic diseases at home and abroad.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Testing food samples to determine whether they contain specific allergens - soy allergen protein test.

[0022] 1. KMnF 4 Nanoparticle preparation: KMnF 4 Nanoparticles can be purchased from the market, and can also be prepared by the following method: Weigh 1.6mmolMnCl 2 4H2O was added to the reaction kettle, then 10mL of absolute ethanol was added, and the solution was stirred until it was completely dissolved; 3 mmol of potassium oleate was weighed and added to the above solution, and stirred for 1-2min to mix well; 5-6g of fluoride Potassium (KF) is ground for more than 10 minutes. When the particle size becomes smaller and finer, weigh 4.98mmol and add it to the previous solution; add absolute ethanol again, usually 30mL; Put the kettle into the oven, adjust the temperature to 160°C, and react for 24 hours; when the reaction kettle drops to room temperature, it usually takes 3-4 hours after closing the oven. Open the reaction kettle, pour out the supernatant, wa...

Embodiment 2

[0030] Determination of whether food samples contain specific allergens—beer foam active protein Z4 allergen detection.

[0031] 1. KMnF 4 Nanoparticle preparation: KMnF 4 Nanoparticles can be purchased from the market, and can also be prepared by the following method: Weigh 1.6mmolMnCl 2 4H2O was added to the reaction kettle, then 10mL of absolute ethanol was added, and the solution was stirred until it was completely dissolved; 3 mmol of potassium oleate was weighed and added to the above solution, and stirred for 1-2min to mix well; 5-6g of fluoride Potassium (KF) is ground for more than 10 minutes. When the particle size becomes smaller and finer, weigh 4.98mmol and add it to the previous solution; add absolute ethanol again, usually 30mL; Put the kettle into the oven, adjust the temperature to 160°C, and react for 24 hours; when the reaction kettle drops to room temperature, it usually takes 3-4 hours after closing the oven. Open the reaction kettle, pour out the super...

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PUM

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Abstract

The invention discloses a KMnF4 nanoprobe-based NMR rapid detection method for food-borne allergens and belongs to the technical field of rapid detection of allergens in food safety. The method depends on an established nuclear magnetic resonance detection process used for allergens in food samples, and detects whether samples contain allergens or not by utilization of influences of paramagnetic characteristics of KMnF4 nanoprobes on nuclear magnetic resonance relaxation time. Specific corresponding relations are that a linear relation exists for the KMnF4 nanoprobes under certain conditions, namely the higher the content of the KMnF4 nanoprobes is, the smaller the spin-lattice relaxation time value of the samples is, so that quantitative detection of the allergens in a certain range can be achieved. The method can be used for rapid detection of specific allergens in food samples so that the method can be used for rapid screening of a large scale of samples to be detected.

Description

technical field [0001] The invention belongs to the technical field of rapid detection of food safety allergens. Relating to a rapid detection method for allergens, in particular to a KMnF-based 4 A rapid NMR method for the detection of food-borne allergens using nanoprobes. Background technique [0002] Food allergens can cause an allergic reaction in infants or young animals that can cause intestinal damage. There are many kinds of food-borne allergens, such as soybean allergen protein, peanut allergen protein, shrimp allergen components, etc., all of which will cause allergic reactions to specific groups of people. At present, there is no radical cure for some allergic diseases at home and abroad. For this reason, patients who are allergic to certain allergens should avoid eating foods containing specific allergens is one of the effective prevention methods at present. In recent years, the food labeling laws of the United States and the European Union stipulate that f...

Claims

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Application Information

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IPC IPC(8): G01N24/08
CPCG01N24/087
Inventor 张锦胜万益琴彭红刘玉环王允圃巫小丹
Owner NANCHANG UNIV
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