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-2459 locus variant of mitochondrial fusion gene 2 and detection method thereof

A site and gene technology, applied in the field of kits for detecting this SNP site, can solve the problems of abnormal expression regulation, less research, and decreased expression, etc.

Inactive Publication Date: 2016-02-24
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

[0005] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

Method used

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  • -2459 locus variant of mitochondrial fusion gene 2 and detection method thereof

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Embodiment 1

[0028] Sequencing was used to detect the 5'non-coding region of the MFn2 gene of essential hypertension susceptibility gene -2459. Thirty samples of the above hypertension case-control group were selected and sequenced to determine the genotype at position -2459 in the 5'non-coding region.

[0029] 1. Experimental method

[0030] PCR sequencing primers still use the above-mentioned PCR primers, and the amplified products are directly sequenced after purification. The sequencing instrument is ABI's 3130xl genetic analyzer, which is analyzed with sequenceanalysis5.2 analysis software, and the results can also be viewed with chromas.

[0031] 2. Experimental results

[0032] Screenshot of sequencing results as figure 1 Shown.

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Abstract

The invention discloses a -2459 locus variant of mitochondrial fusion gene 2 and a detection method thereof, discloses a single nucleotide locus related to primary hypertension susceptibility and discloses a method for detecting single nucleotide polymorphism of primary hypertension related gene-mitochondrial fusion gene 2 (Mfn2) or hyperplasia suppressor gene (HSG). The method includes: detecting genotype of a -2459 locus in a 5' end non-coding region of the mitochondrial fusion gene 2 (Mfn2) or hyperplasia suppressor gene (HSG) of an individual. The invention further discloses a corresponding detection kit.

Description

Technical field [0001] The invention relates to the fields of molecular biology and medicine. More specifically, it relates to a single nucleotide polymorphism (Singlenucleotidepolymorphism, SNP) at position -2459 in the 5'non-coding region of human mitochondrial fusion gene 2 (Mitofusin2, Mfn2gene) and its correlation with essential hypertension Detection. The present invention also relates to a kit for detecting this SNP site. Background technique [0002] Essential hypertension (EH) is a common and frequently occurring multi-factor and multi-gene disease caused by environmental and genetic factors, which has a great impact on human health. With the development of molecular medicine, there are more than 150 hypertension-related genes that have been discovered, but the pathogenesis of EH is still not fully understood, and the early diagnosis and prevention of hypertension have not been completely resolved. EH is the result of the combination of genetic factors and the environ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 王佐广彭晓云魏永祥刘雅靳飞刘洁琳孙东东李梅牛秋丽李闯温绍君
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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