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Variation site of hypertension susceptibility gene Mfn2 and detecting method of variation site

A technology of essential hypertension and loci, applied in the fields of molecular biology and medicine, can solve the problems of decreased expression, abnormal expression regulation, and less research.

Inactive Publication Date: 2016-02-24
BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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Problems solved by technology

[0005] Since the main characteristic of Mfn2 gene in the blood vessels and leukocytes of patients with essential hypertension and spontaneously hypertensive rats is the decreased expression, this should be due to the abnormality of its expression regulation. few

Method used

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  • Variation site of hypertension susceptibility gene Mfn2 and detecting method of variation site

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Embodiment 1

[0028] The region between -2517 and -2518 of the 5' non-coding region of the essential hypertension susceptibility gene MFn2 gene was detected by sequencing. 30 cases of each of the above-mentioned hypertension cases and control groups were selected for sequencing to determine the genotype of the region between -2517 and -2518 in the 5' non-coding region.

[0029] 1. Experimental method

[0030] The above PCR primers were still used as primers for PCR sequencing, and the amplified products were directly sequenced after purification. The sequencing instrument is ABI's 3130xl genetic analyzer, which is analyzed with sequenceanalysis5.2 analysis software, and the results can also be viewed with chromas.

[0031] 2. Experimental results

[0032] The screenshot of the sequencing result is as follows: figure 1 shown.

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Abstract

The invention discloses a single nucleotide site related to essential hypertension susceptibility and a method for detecting single nucleotide polymorphism of mitofusin 2 (Mfn2) / hyperplasia suppressor gene (HSG) which is related to essential hypertension. The method includes: detecting the genotype of a region between the 5'non-coding region-2517 site and the 5'non-coding region-2518 site of single Mfn2 and HSG. The invention further discloses a corresponding detection kit.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine. More specifically, it involves the single nucleotide polymorphism (Singlenucleotidepolymorphism, SNP) between the -2517 and -2518 sites in the 5' non-coding region of the human mitochondrial fusion gene 2 (Mitofusin2, Mfn2gene) and its relationship with primary Hypertension-associated detection. The invention also relates to a kit for detecting the SNP site. Background technique [0002] Essential hypertension (Essential hypertension, EH) is a common and frequent multifactorial and polygenic disease caused by environmental and genetic factors, which has a great impact on human health. With the development of molecular medicine, more than 150 hypertension-related genes have been discovered, but the pathogenesis of EH is still not completely clear, and the early diagnosis and prevention of hypertension have not been fully resolved. EH is the result of the joint action of ge...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6883C12Q2600/156
Inventor 魏永祥王佐广李梅彭晓云靳飞刘洁琳刘雅牛秋丽李闯孙东东温绍君
Owner BEIJING ANZHEN HOSPITAL AFFILIATED TO CAPITAL MEDICAL UNIV
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