Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation and application of Al<3+> fluorescent probe based on rhodamine B derivative

A technology of fluorescent probes and derivatives, applied in the direction of fluorescence/phosphorescence, luminescent materials, chemical instruments and methods, etc., to achieve high yield, low detection limit, and good selectivity

Inactive Publication Date: 2016-02-24
HAINAN MEDICAL COLLEGE
View PDF5 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But on Al 3+ There are few reports on detection

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of Al&lt;3+&gt; fluorescent probe based on rhodamine B derivative
  • Preparation and application of Al&lt;3+&gt; fluorescent probe based on rhodamine B derivative
  • Preparation and application of Al&lt;3+&gt; fluorescent probe based on rhodamine B derivative

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Synthesis of rhodamine B derivative P compound shown in formula 1 (see figure 1 ):

[0023] 1mmol rhodamine B hydrazinolysis product (X.F.YangX.Q.Guo, Y.B.Zhao, Talanta, 2002, 57, 883890.) and 5mmol glyoxal in absolute ethanol, stirred at room temperature for 6-8h, filtered, and then used Wash with absolute ethanol and dry to obtain the intermediate compound.

[0024] Then the obtained intermediate was reacted with o-aminophenol in absolute ethanol at 80°C for 4 hours at a molar ratio of 1:1. Petroleum ether-ethyl acetate is used as eluent to obtain yellow solid P through chromatographic column separation; Wherein, petroleum ether:ethyl acetate (v:v)=5:1, productive rate 85% (see figure 2 and 3 ).

[0025] From figure 2 Molecular ion peaks and image 3 The chemical shift of H in can be used to know the formation of rhodamine B derivative P.

[0026] Rhodamine B derivatives (P) to Al 3+ optical recognition

[0027] 1) The diluent is: in ethanol / water (9 / 1, v / v...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a fluorescent molecular probe and particularly to preparation and application of a rhodamine B derivative. A preparation method comprises the following steps of enabling a rhodamine B hydrazinolysis product to react with glyoxal in anhydrous ethanol at the room temperature for 6-8 hours, and precipitating a solid to obtain an intermediate compound; and enabling the intermediate to react with ortho-aminophenol in the anhydrous ethanol at the temperature of 70-80 DEG C for 4-6 hours, cooling down the reaction product, then carrying out suction filtration on the precipitated solid, and performing washing, drying and separating to obtain a yellow solid P as shown in a formula 1. The rhodamine B derivative P can be used as a fluorescent probe to implement identification and detection on aluminum ions. According to the present invention, the rhodamine B derivative P obtained by an effective combination means shows good selectivity for Al<3+>, and on the basis of optimizing an experimental condition, detection on the aluminum ions can be implemented. The formula 1 is shown as the specification.

Description

technical field [0001] The invention relates to a fluorescent molecular probe, in particular to the preparation and application of a rhodamine B derivative. Background technique [0002] In the reported aluminum ion fluorescent probes, most of the fluorescence peaks are at short wavelengths, which cannot be observed by naked eyes (F.Dai, F.Liu, Q.Luo, C.W.YuandQ.L.Huang, International Journal of Science and Technology, 2014, 3, 1 -4.; J. Zhang, N. Li, F. Dai, Q. Luo and Y. X. Ji, Sensors & Transdusors, 2015, 186, 125-128.; S. B. Wen, Y. X. Ji, C. X. Guan, and Q. Y. Fu, Sensors & Transdusors, 2015,190,31-34.), this pair of Al 3+ The detection has brought great influence and inconvenience. Especially due to the influence of the coordination properties of aluminum ions themselves, Al 3+ There are fewer studies on probes (A. Banerjee, A. Sahana, S. Das, S. Lohar, S. Guha, B. Sarkar, S. K. Mukhopadhyay, A. K. Mukherjee and D. Das, Analyst, 2012, 139, 2166-2175.) . [0003] R...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/107C09K11/06G01N21/64
CPCC07D491/107C09K11/06C09K2211/1088G01N21/6402
Inventor 于春伟张军
Owner HAINAN MEDICAL COLLEGE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com