Preparation and application of benzoyl hydrazine derivative

Abstract

The present invention relates to a fluorescent molecular probe, and particularly to preparation and application of a benzoyl hydrazine derivative . The structure of the benzoyl hydrazine derivative P is as shown in a formula 1. A preparation method comprises the following steps: heating ethyl anthranilate and hydrazine hydrate to reflux in anhydrous alcohol for 4-6 h, cooling down the mixture until the temperature reaches the room temperature, then precipitating and filtering a solid, and washing the solid with anhydrous alcohol , and drying to obtain an intermediate compound; and enabling the intermediate to react with 2-hydroxy-1-naphthaldehyde for 4-6 hours in anhydrous alcohol at a temperature of 70-80 DEG C, cooling down the mixture, then precipitating and leaching a solid, then washing the solid, and drying the solid to obtain a yellowish solid pure product P. The benzoyl hydrazine derivative P can be used as a fluorescent probe to implement identification and detection on Mg2+. The benzoyl hydrazine derivative P obtained by an effective synthesis means has relatively good selectivity on Mg2+, and on the basis of optimized experimental conditions, detection on Mg2+ can be implemented. The formula 1 is as shown in the specification.

Description

technical field [0001] The invention relates to a fluorescent molecular probe, in particular to the preparation and application of a benzohydrazide derivative. Background technique [0002] Among the reported fluorescent molecular probes, most of them have the following disadvantages: complex synthesis and separation, large interference of other coexisting ions in the test process, high detection limit, and quenching response signal, etc. (Y.T.LiandC.M.Yang, Chem. .Commun.,2003,2884-2885.;L.Fabbrizzi,M.Licchelli,P.Pallavicini,A.Perotti,A.TagliettiandD.Sacchi,Chem.Eur.J.,1996,2,75-82.;M.N. Elizabeth, J.L.Stephen, J.Mater.Chem., 2005,15,2778-2783.), which has brought great influence and inconvenience to the detection of metal ions. especially due to the influence of Ca 2+ interference, Mg 2+ There are few studies on probes (Zhang Dengqing. Advances in Chemistry, 2009, 21, 715-723.). [0003] Benzohydrazide derivatives have excellent optical properties and are easy to synth...

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Problems solved by technology

[0002] Among the reported fluorescent molecular probes, most of them have the following disadvantages: complex synthesis and separation, large interference of other coexisting ions in the test process, high detection limit, and quenching response signal, etc. (Y.T.LiandC.M.Yang, Chem. .Commun.,2003,2884-2885.;L.Fabbrizzi,M.Licchelli,P.Pallavicini,A.Perotti,A.TagliettiandD.Sacchi,Chem.Eur.J.,1996,2,75-82.;M.N. Elizabeth, J.L.Stephen, J.Mater.Chem., 2005,15,2778-2783.), which has brought great influence and inconvenience to the detection of metal ions

but on Mg 2+ There are few reports on detection

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