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Preparation method of antineoplastic drug carrier having dual-lymphatic targeting

An anti-tumor drug and lymphatic targeting technology, which is applied in the direction of anti-tumor drugs, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., to achieve high targeting rate, stable physical and chemical properties, and high drug loading rate effect

Inactive Publication Date: 2016-02-24
SHANGHAI JIAO TONG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to statistics, clinically, 60% of malignant tumor patients have already developed lymphatic metastasis when they are first diagnosed, and there is currently no effective means to control and treat them.

Method used

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  • Preparation method of antineoplastic drug carrier having dual-lymphatic targeting
  • Preparation method of antineoplastic drug carrier having dual-lymphatic targeting
  • Preparation method of antineoplastic drug carrier having dual-lymphatic targeting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] a. Add 8 parts of chitosan to 4 parts of 1% acetic acid solution and stir for 1 hour. After the chitosan is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers in turn.

[0035] b. Add 5 parts of sodium tripolyphosphate to 4 parts of deionized water, and stir for 30 minutes. After the sodium tripolyphosphate is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers in turn.

[0036] c. Add 4 parts of paclitaxel to 5 parts of methanol and stir for 15 minutes until the drug is completely dissolved.

[0037] d. Add 5 parts of paclitaxel methanol solution dropwise to 5 parts of chitosan solution, and then slowly drop 2 parts of sodium tripolyphosphate solution into the solution under continuous magnetic stirring, and stir for 30 minutes to form a paclitaxel-loaded shell Glycan nanoparticles. Then centrifuge at 12000 rev / min, and freeze-dry the extract in a lyophilizer to obtain paclitaxel-loaded chitosan nanoparticle...

Embodiment 2

[0041] a. Add 10 parts of chitosan to 5 parts of 1% acetic acid solution and stir for 1.5 hours. After the chitosan is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers in turn.

[0042] b. Add 4 parts of sodium tripolyphosphate to 4 parts of deionized water, and stir for 30 minutes. After the sodium tripolyphosphate is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers in turn.

[0043] c. Add 4 parts of paclitaxel to 4 parts of methanol solution and stir for 10 minutes until the drug is completely dissolved.

[0044] d. Add 5 parts of paclitaxel solution dropwise to 5 parts of chitosan solution, under continuous magnetic stirring, slowly add 2 parts of sodium tripolyphosphate solution into the solution, and stir for 30 minutes to form paclitaxel-loaded chitosan Nanoparticles. Then centrifuge at 11000 rev / min, and freeze-dry the extract in a lyophilizer to obtain paclitaxel-loaded chitosan nanoparticle powder.

...

Embodiment 3

[0048] a. Add 9 parts of chitosan to 5 parts of 1% propionic acid solution, stir for 1 hour, after the chitosan is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers successively.

[0049] b. Add 5 parts of sodium tripolyphosphate to 4 parts of deionized water, and stir for 30 minutes. After the sodium tripolyphosphate is completely dissolved, pass the solution through 0.45 μm and 0.22 μm filter papers in turn.

[0050] c. Add 5 parts of doxorubicin to 4 parts of ethanol solution and stir for 20 minutes until the drug is completely dissolved.

[0051] d. Add 6 parts of doxorubicin solution dropwise to 5 parts of chitosan solution, and then slowly add 2 parts of sodium tripolyphosphate solution into the solution under continuous magnetic stirring, and stir for 30 minutes to form loaded doxorubicin chitosan nanoparticles. Then centrifuge at 10,000 revs / min, and freeze-dry the extract in a lyophilizer to obtain chitosan nanoparticle powder of doxor...

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Abstract

The invention discloses a preparation method of an antineoplastic drug carrier having dual-lymphatic targeting. The preparation method comprises the following steps: preparing drug-loaded chitosan nanoparticles: adding an antineoplastic drug solution to a chitosan solution and stirring; and adding a sodium tripolyphosphate solution and stirring, so as to obtain the drug-loaded chitosan nanoparticles; and preparing the dual-lymphatic targeting antineoplastic drug carrier: dispersing the drug-loaded chitosan nanoparticles in water, adding a cross-linking agent, sodium hyaluronate and LyP-1 and stirring so as to obtain the dual-lymphatic targeting antineoplastic drug carrier. The preparation method disclosed by the invention is simple in process, and the method is capable of achieving massive preparation in one time with a high drug-loading capacity. The prepared antineoplastic drug carrier is stable in physical and chemical properties and is capable of keeping a uniform morphology for a long time without agglomeration. The antineoplastic drug carrier is high in tumor targeting rate, and is capable of accurately positioning and retaining in lymphatic metastatic tumor and releasing antineoplastic drugs therein.

Description

technical field [0001] The invention relates to the field of preparation of nanomaterials, in particular to a method for preparing an antitumor drug carrier with double lymphatic targeting. Background technique [0002] Lymphatic metastasis is an important form of metastasis of solid tumors, and it is also an important cause of tumor death in patients. According to statistics, clinically, 60% of malignant tumor patients have already developed lymphatic metastasis when they are first diagnosed, and there is no effective means to control and treat it. Therefore, applied basic research on targeted therapy for lymphatic metastatic tumors has important clinical significance. [0003] Hyaluronic acid (HA) is the only component of the extracellular matrix and interstitium, and plays an important role in maintaining the structure of the extracellular matrix and regulating intracellular activities. HA not only has good biocompatibility, degradability, high viscoelasticity and non-i...

Claims

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Application Information

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IPC IPC(8): A61K9/19A61K45/00A61K31/337A61K31/704A61K47/36A61K47/42A61P35/00
Inventor 何丹农乔宇易帆姬豫朱君金彩虹
Owner SHANGHAI JIAO TONG UNIV
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