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Portable C reaction protein detection kit

A detection kit and reactive protein technology, applied in the field of biomedicine, can solve problems such as false positives, easy to be copied and tampered, complicated operation process, etc., and achieve the effect of improving the simplicity of operation and avoiding copying and tampering

Inactive Publication Date: 2016-02-17
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection process needs to read the ID chip data and time the reaction twice, the operation process is complicated, and the ID chip has no encryption function, so it is easy to be copied and tampered with; moreover, this method is also susceptible to the interference of rheumatoid factor and heterophile antibody, resulting in false positives

Method used

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  • Portable C reaction protein detection kit
  • Portable C reaction protein detection kit
  • Portable C reaction protein detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Preparation of C-reactive protein detection kit

[0049] 1) Preparation of fluorescent tracer-labeled antibody

[0050] Dilute the carboxy fluorescent microspheres to a concentration of 5mg / ml with 10mmol / L, pH=6.5 phosphate buffer solution to prepare a microsphere suspension, add 5mg of EDAC to each 10ml microsphere suspension, mix well, and let it stand at room temperature. Place for 1 hour, centrifuge at 10000rpm for 20min, remove the supernatant (supernatant), suspend the pellet in an equal volume of 10mmol / L, pH=7.2 phosphate buffer, and ultrasonically disperse; then every 10ml of carboxyl microspheres after ultrasonic dispersion Add 3 mg of C-reactive protein monoclonal antibody 1 (monoclonal antibody to the first epitope of C-reactive protein; for the fusion of splenocytes and bone marrow cells obtained from mice immunized with C-reactive protein, and obtain the ability to permanently produce C-reactive protein through screening. The hybridoma cells o...

Embodiment 2

[0068] Example 2: Performance evaluation of C-reactive protein detection kit

[0069] 1) Comparative experiment

[0070] The kit of the present invention and the specific protein meter of beckmanIMMAGE800 detect 243 samples of C-reactive protein in the range of 0.5-200 mg / L (whole blood / plasma) at the same time, as attached Figure 5 , the result is that the correlation coefficient R2 of the two is 0.987.

[0071] 2) Interfering experiments

[0072] After adding different amounts of interfering substances to the same human serum sample, the determination is carried out. The difference between the measured value after adding the interfering substance and the measured value before adding the interfering substance divided by the ratio of the measured value before adding the interfering substance is the interference rate. The test results show that the concentrations of human rheumatism factor and human anti-mouse antibody are respectively When they are below 1200IU / mL and 6.5m...

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Abstract

A portable C reaction protein detection kit comprises a test strip, an upper clamp slot, a lower clamp slot and a radio frequency identification chip; the test strip comprises a plastic cement plate, on which a sample pad, a first antibody bearing pad, a cellulose membrane and a water absorption pad are arranged in sequence; the first antibody bearing pad and the water absorption pad are respectively overlapped at two ends of the cellulose membrane; one end of the sample pad is overlapped on the first antibody bearing pad; the cellulose membrane is provided with a detection belt T for fixing a C reaction protein second antibody and a quality control belt for fixing a sheep anti-mouse antibody; the test strip is arranged in a cavity formed by splicing the upper clamp slot and the lower clamp slot; the upper clamp slot is provided with a sampling hole and a detection window; a sample diluent is a buffer solution containing a rheumatoid factor and heterophil antibody blocking agent; the lower clamp slot is provided with the radio frequency identification chip close to the left end. The kit has the advantages of being capable of accurately reflecting antibody activity, capable of avoiding wrong detection results, simple to use, and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a convenient C-reactive protein detection kit. Background technique [0002] At present, quantitative immunoassay technology mainly includes the following quantitative detection methods: [0003] One is the immunoturbidimetric detection technology supporting large-scale, highly integrated automated instruments; [0004] The second is enzyme-linked immunochromatography detection technology; [0005] The third is the rapid detection technology of immunochromatography; [0006] Among them, immunoturbidimetric detection technology and enzyme-linked immunoassay technology have disadvantages such as sample pretreatment, complicated operation, long detection time, complex large-scale equipment, poor mobility, and long reporting time, which are more suitable for centralized detection of large batches of samples. , Not suitable for rapid detection in emergency departmen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/544
CPCG01N33/533G01N33/544
Inventor 邹炳德邹继华方亮刘献文
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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