Efficient denitrification novel Acinetobacter and application thereof
An Acinetobacter, high-efficiency technology, applied in the field of microorganisms, can solve problems such as nitrite ammonia nitrogen exceeding the standard, and achieve the effect of protecting the environment and improving the ability of denitrification
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Embodiment 1
[0017] The acquisition of embodiment 1 Acinetobacter new bacteria
[0018] 1.1 Prepare the following medium:
[0019] Ammonia oxidation medium: NH 4 Cl0.382g, sodium acetate 2g, MgSO 4 7H 2 O0.05g, K 2 HPO 4 0.2g, NaCl0.12g, MnSO 4 4H 2 O0.01g, FeSO 4 0.01g, dilute water to 1000mL, pH7.2;
[0020] Nitrite reducing medium: NaNO 2 0.04928g, glucose 10g, sodium acetate 1g, Na 2 HPO 4 3g, KH 2 PO 4 2g, NaCl3g, water 1L, pH7.2; add 1.5% agar to the solid medium.
[0021] 1.2 Screening of heterotrophic nitrifying bacteria
[0022] 1. Collect samples : The samples in the experiment were collected from the pond bottom mud in Nansha District, Guangzhou City, Guangdong Province.
[0023] 2. Microbial enrichment culture:
[0024] (1) Take 20mL of the collected fresh sample and add it to 180mL of ammonia oxidation medium. After enrichment culture at 30°C and 180r / min for 3 days, repeat the enrichment once to obtain the enrichment solution, which is set aside.
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Embodiment 2
[0028] Embodiment 2: the characteristic of novel microorganism of the present invention
[0029] 2.1 Identification of strain morphological characteristics
[0030] Take a single colony of strain YZS-X1-1, transfer it to LB solid medium (agar), and culture it in a constant temperature incubator at 30°C for 24h, 36h, and 48h, and observe the size, color, edge, and protrusion of the colony respectively , smoothness, viscosity, transparency and other characteristics. The results showed that strain YZS-X1-1 formed neat, smooth, viscous, glossy and opaque colonies on LB solid medium. Observed under the microscope, it is short rod-shaped, and the result is as follows figure 1 shown.
[0031] 2.2 Physiological and biochemical characteristics of the strain
[0032] Pick fresh cultures cultured on LB solid medium (agar) for 24 hours for physiological and biochemical tests. The results showed that YZS-X1-1 was a Gram-negative bacillus.
[0033] 1. Identification characteris...
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