ABA (abscisic acid) concentration detection method based on biosensing technology

Abstract

The invention discloses an ABA (abscisic acid) concentration detection method based on the biosensing technology. The method can achieve quick detection of the ABA concentration in a to-be-detected organ under the condition that the to-be-detected organ of a to-be-detected plant is not damaged. The method comprises the following steps: S1, integrating a platinum counter electrode, a silver / silver chloride reference electrode and a modified gold working electrode into a microelectrode array, wherein the modified gold working electrode is obtained through sequentially modifying a gold working electrode with thionine, gold nanoparticles and ABA antibody; the platinum counter electrode, the silver / silver chloride reference electrode and the modified gold working electrode are connected with an electrochemical workstation; S2, putting the detection end of the electrode on the microelectrode array into the to-be-detected part of the to-be-detected plant, collecting the impedance generated by the working electrode through using the electrochemical workstation and adopting the alternating current impedance method, calculating the average value of the impedance in preset d uration after the impedance change becomes steady, and substituting the average value into a precomputed linear formula of the ABA concentration and the average value of impedance, so as to obtain the ABA concentration of the to-be-detected part.

Description

technical field [0001] The invention relates to the field of plant ABA detection, in particular to an ABA concentration detection method based on biosensing technology. Background technique [0002] Water stress is an important environmental factor that affects plant growth and development and limits crop yield. Abscisic acid (ABA) plays an important role in regulating plant response to water stress. ABA can not only regulate plant water metabolism by inducing stomatal closure, but also enhance plant resistance to water stress by inducing the expression of dehydration tolerance proteins. [0003] The detection of common plant ABA mainly relies on bioassay, gas chromatography, enzyme-linked immunoassay, radioimmunoassay and high performance liquid chromatography. Although biologists have made it clear that ABA has been acting as a growth-regulating substance long ago, relatively speaking, we know little about the essence of the physiological and biochemical effects of ABA. ...

AI Technical Summary

Problems solved by technology

[0004] However, traditional detection methods require cumbersome pretreatments such as separation, extraction, and derivatization of the sample, which not only has problems such as time-consuming and high cost, but more importantly, the pretreatment will cause irreversible damage to the sample material.

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