A super-resolution structure detection confocal fluorescence imaging device and imaging method thereof

Abstract

The invention provides a super-resolution structure detection confocal fluorescence imaging device and an imaging method thereof, relating to imaging devices and imaging methods thereof and aiming to solve the problems that the resolution of the existing confocal limiting technology is difficult to increase and confocal images are not clear. The device comprises a laser source, wherein a collimator and beam expander, a beam splitter prism, a 1 / 4 wave plate, a scanning system, an illumination objective, a fluorescent sample, a collecting lens and a CCD (charge coupled device) detector are arranged along the ray propagation direction of the laser source in sequence. The device has the effects that integration is carried out on a detection plane to change the light sensitivity of a corresponding detecting location; the OTF (optical transfer function) bandwidth of a system is expanded; the spatial cut-off frequency of a confocal fluorescence imaging system is improved and the spatial frequency domain bandwidth is expanded, thus obviously improving the lateral resolution of the imaging system; and the device is applicable to the measurement field of thick biological sample imaging.

Description

technical field [0001] The invention relates to an imaging device and an imaging method thereof, in particular to a super-resolution structure detection confocal fluorescence imaging device and an imaging method thereof, belonging to the technical field of optical precision measurement. Background technique [0002] Optical microscopy is a long-established and important non-destructive technique widely used in fields such as biology and materials science. Confocal microscopic measurement technology is a three-dimensional optical microscopy technology suitable for micron and submicron scale measurement. The tomographic capabilities of reflective confocal microscopy systems make them important in the field of 3D imaging. [0003] In the mid-to-late 1950s, the confocal microscope was invented by Minsky. In 1977, C.J.R. Sheppard and A.Choudhury first clarified that the confocal microscope system under the action of the dot pinhole mask, at the expense of the field of view, the ...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that the resolution of the existing confocal microscopy technology is difficult to improve and the confocal imaging is not clear

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