A special enzyme for suckling pig containing mold culture and its preparation method
A technology for mold culture and suckling piglets, which is applied in the field of special enzymes for suckling piglets and its preparation, can solve the problems that the function of the culture and the preparation method need to be improved, the activity of the enzyme preparation needs to be continuously improved, and the special enzyme enzyme system is incomplete, etc., to achieve the purpose of regulating stomach The effect of intestinal flora balance, inhibition of growth and reproduction, and promotion of animal growth
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Embodiment 1
[0060] Embodiment 1 raw material preparation
[0061] Preparation of mold cultures:
[0062] The preparation method of described mold culture comprises the following steps:
[0063] (1) Activation of bacteria
[0064] Inoculate the well-preserved slant strain of Aspergillus Usami CCTCC NO: M 2013601 on the slant medium, culture at 32°C for 40 hours to activate the strain, and activate it 3 times;
[0065] (2) Expanded cultivation of liquid seeds
[0066] ① First-level seed cultivation: Wash the spores of the slanted bacteria after activation in step (1) with sterile water, insert them into a 500-ml shaker flask, fill the liquid seed medium with 100 ml, and culture on a shaker at 32°C and 100 rpm for 40 hours;
[0067] ②Cultivation of secondary seeds: Inoculate 10% of the primary seeds into 500 ml secondary seed shake flasks, and the cultivation conditions are the same as those of the primary seeds;
[0068] ③Third-level seed cultivation: insert the second-level seeds into ...
Embodiment 2
[0085] A special enzyme for suckling piglets containing mold culture, consisting of the following raw materials in parts by weight:
[0086] 42 parts of mold culture, 40 parts of plant extract, 35 parts of amylase, 35 parts of acid xylanase, 25 parts of cellulase, 25 parts of beta-glucanase, 18 parts of pectinase, 12 parts of mannose 12 parts of phytase, 8 parts of Chinese herbal medicine extract, 7 parts of protective agent, 7 parts of activator, 4 parts of antioxidant;
[0087] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;
[0088] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0089] Described protecting agent is made up of the raw material of following parts by weight: trehalose 25 parts, NaCl 25 parts, (NH 4 ) 2 SO 4 12 parts, cysteine 12 parts;
[0090] The activator is formed by uniformly mixing inorganic salts with the following mas...
Embodiment 3
[0095] A special enzyme for suckling piglets containing mold culture, consisting of the following raw materials in parts by weight:
[0096] 35 parts of mold culture, 35 parts of plant extract, 30 parts of amylase, 30 parts of acid xylanase, 20 parts of cellulase, 20 parts of beta-glucanase, 15 parts of pectinase, 10 parts of mannose 10 parts of phytase, 6 parts of Chinese herbal medicine extract, 5 parts of protective agent, 5 parts of activator, 3 parts of antioxidant;
[0097] The mold culture, plant extract, and Chinese herbal medicine extract are all prepared in Example 1;
[0098] The acid xylanase, cellulase, β-glucanase, pectinase, amylase, mannase, and phytase are all food-grade enzyme preparations;
[0099] Described protecting agent is made up of the raw material of following parts by weight: trehalose 20 parts, NaCl 20 parts, (NH 4 ) 2 SO 4 10 parts, cysteine 10 parts;
[0100] The activator is formed by uniformly mixing inorganic salts with the following m...
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