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Detection method for microalbuminuria (mAlb)

A urine microalbumin and detection method technology, applied in measuring devices, biological testing, material inspection products, etc., can solve problems such as radioactive contamination, poor repeatability, and short shelf life of reagents

Inactive Publication Date: 2016-02-03
王贤俊
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Radioimmunoassay has high sensitivity and strong specificity, but has the disadvantages of radioactive contamination and short shelf life of reagents.
At present, the most widely used detection method for urine microalbumin in China is still the ELISA method, but the enzyme-labeled method has many steps, so the repeatability is poor

Method used

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  • Detection method for microalbuminuria (mAlb)
  • Detection method for microalbuminuria (mAlb)
  • Detection method for microalbuminuria (mAlb)

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Experimental program
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Embodiment

[0016] The inventive method is mixed with reagent, carries out performance comparison with commercially available reagent:

[0017] The reagent is a double reagent, including reagents R1 and R2, and the specific components are as follows:

[0018]

[0019] Use of urine microalbumin detection reagents:

[0020] 1) Detection instrument: a biochemical analyzer with a wavelength of 340nm and a constant temperature device at 37°C.

[0021] 2) Sample to be tested: urine, stable for one day at 2-8°C, preferably morning urine, centrifuged (3000rpm / min) for 10 minutes before use.

[0022] 3) Determination of basic parameters:

[0023] dominant wavelength 340nm temperature reflex 37℃ analysis type endpoint method Reagent to sample ratio 20∶1 Reaction time 10 minutes Calibration type Logit-4P / Spline

[0024] 4) Specific testing procedures:

[0025]

[0026] 5) Calculation result: mAlb in the sample (mg / L) = Cs × ΔA T / ΔA s

[0027] Whe...

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Abstract

The invention provides a detection method for microalbuminuria (mAlb). The detection method for microalbuminuria is based on the latex-enhanced immunoturbidimetric assay, and adopts a liquid double reagent comprising a reagent R1 and a reagent R2, wherein the reagent R1 serves as a reactant; the reagent R2 is a solution containing albumin immuno-latex particles. The detection method is characterized by applying a chemical method for cross-linking and preparing an mAlb antibody latex reagent via covalent cross-linking between goat anti-human albumin antibody (also called as goat anti-human mAlb antibody) and carboxylated polystyrene by using water-soluble carbodiimide (EDC) and N-hydroxy succinimide (NHS). The mAlb antibody latex reagent is high in sensitivity and specificity, simple to prepare, and worth of further popularization and use.

Description

Technical field: [0001] The invention belongs to the field of biotechnology and provides a quantitative detection method for urinary microalbumin (mAlb). Background technique: [0002] Microalbuminuria is the presence of tiny amounts of albumin in the urine. Albumin is a normal protein in the blood, but only very small amounts are present in the urine under physiological conditions. Microalbuminuria reflects abnormal protein leakage from the kidneys. Increased urinary microalbumin is more common in diabetic nephropathy, hypertension, and preeclampsia, and is an early sensitive indicator of kidney damage. Urinary microalbumin caused by any disease is due to the damage of the intrinsic cells of the kidney due to different initial causes, which changes the structure of the intrinsic cells of the kidney, and the function changes with the change of the structure, which is reflected in the urine. When the urinary microalbuminuria is found to be in the range of 20mg / L-200mg / L, a...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/6827G01N2333/76
Inventor 王贤俊郑蓓蕾郭二豪江新涛
Owner 王贤俊
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