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Spriulina polysacchride and preparation method thereof

A technology of spirulina polysaccharides and spirulina, which is applied in the field of natural medicine extraction and preparation, can solve the problems of no single polysaccharide extraction and application research, limitations, etc., and achieve the effect of inhibiting the proliferation of human liver cancer cells

Active Publication Date: 2016-02-03
SHENZHEN NEPTUNUS PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There have been many reports on Spirulina polysaccharides, but most of them are limited to crude polysaccharides. At present, there is no literature on the extraction and application of single polysaccharides in Spirulina.

Method used

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  • Spriulina polysacchride and preparation method thereof
  • Spriulina polysacchride and preparation method thereof
  • Spriulina polysacchride and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: a kind of preparation method of spirulina polysaccharide, specifically:

[0024] Take 2Kg of spirulina, add 40L of 0.5mol / L NaOH solution, stir and extract at a solution temperature of 60°C for 2 hours; after cooling, add 6mol / L HCl solution to adjust the pH value to 2.5; centrifuge to obtain a supernatant. Concentrate the supernatant to about 5 L, add 15 L of 95% edible ethanol, let it stand for 10 hours, and collect the precipitate by centrifugation to obtain 300 g of spirulina crude polysaccharide.

[0025] Take 200g of the above-mentioned spirulina crude polysaccharide, add 6L of water to dissolve; in a water bath at 35°C, add 0.5mol / LNaCl, 0.5mol / LNaAc; after 4.0mmol / L acetic acid; add 2L of 10% H2O2 dropwise at a constant speed; wait for 4 hours to react, and the reaction is complete Afterwards, 150 g of spirulina homogeneous polysaccharides were obtained by ultrafiltration. It is detected as a homogeneous polysaccharide by high performance gel chro...

Embodiment 2

[0026] Embodiment 2: a kind of preparation method of spirulina polysaccharide, specifically:

[0027] Take 2Kg of spirulina, add 20L of 0.5mol / L NaOH solution, stir and extract at a solution temperature of 60°C for 2 hours; after cooling, add 6mol / L HCl solution to adjust the pH value to 2; centrifuge to obtain a supernatant. Concentrate the supernatant to about 5 L, add 15 L of 95% edible ethanol, let it stand for 11 hours, and collect the precipitate by centrifugation to obtain 270 g of spirulina crude polysaccharide.

[0028] Take 200g of the above-mentioned spirulina crude polysaccharide, add 4L of water to dissolve; in a water bath at 35°C, add 0.5mol / LNaCl, 0.5mol / LNaAc; after 4.0mmol / L acetic acid; add 1L of 10% H2O2 dropwise at a constant speed; react for 4 hours, wait for the reaction to end Afterwards, ultrafiltration was carried out to obtain 143 g of spirulina homogeneous polysaccharide. It is detected as a homogeneous polysaccharide by high performance gel chroma...

Embodiment 3

[0029] Embodiment 3: a kind of preparation method of spirulina polysaccharide, specifically:

[0030] Take 2Kg of spirulina, add 60L of 0.5mol / L NaOH solution, stir and extract at 60°C for 2 hours; after cooling, add 6mol / L HCl solution to adjust the pH to 3; centrifuge to obtain the supernatant. Concentrate the supernatant to about 5 L, add 15 L of 95% edible ethanol, let it stand for 12 hours, and collect the precipitate by centrifugation to obtain 313 g of spirulina crude polysaccharide.

[0031] Take 200g of the above-mentioned spirulina crude polysaccharide, add 8L of water to dissolve; in a water bath at 35°C, add 0.5mol / LNaCl, 0.5mol / LNaAc; 4.0mmol / L acetic acid; add 3L of 10% H2O2 dropwise at a constant speed; , carried out ultrafiltration to obtain 144 g of spirulina homogeneous polysaccharide. It is detected as a homogeneous polysaccharide by high performance gel chromatography (HPGPC), and its weight average molecular weight is 9500Da.

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Abstract

The invention discloses a spriulina polysacchride with the antitumor activity, and a preparation method of the spriulina polysacchride. A derivative of the spriulina polysacchride comprises four monosaccharides of D-glycuronic acid, D-acetylgalactosamine, L-fucose and galactose, and the molecular weight of the derivative is 5000-18000 Da. The extracted single spriulina polysacchride has an obvious inhibiting effect on tumor cells, and thus can be used for preparation of antitumor drugs.

Description

technical field [0001] The invention belongs to the field of extraction and preparation of natural medicines, and more specifically relates to a spirulina polysaccharide and a preparation method thereof. Background technique [0002] Spirulina is a class of lower organisms of Cyanophyceae, Oscillatoriaceae, prokaryotic organisms, filamentous bodies composed of single cells or multi-cells, body length 200-500 μm, width 5-10 μm, cylindrical, loose or compact There is a regular spiral bend, shaped like a clockwork spring, hence the name. Spirulina has various functions such as lowering cholesterol, regulating blood sugar, enhancing immunity, protecting gastrointestinal function, anti-tumor, anti-oxidation, anti-aging, anti-fatigue, and anti-radiation. [0003] Spirulina is characterized by protein content as high as 60%-70%, low fat and cellulose content, and a wide variety of vitamins. It is the food with the highest content of vitamin B12 and β-carotene. In addition, it is a...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P35/00
Inventor 谭道鹏冯贻东于琳王春宴曾伟珍
Owner SHENZHEN NEPTUNUS PHARM CO LTD
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