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Method for extracting branched chain amino acid from fermentation liquid by virtue of double-membrane continuous crystallization method

An amino acid, fermentation broth technology, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of poor product quality, large waste water discharge, low product yield, etc., and achieve good quality and product quality. The effect of improving and high product yield

Active Publication Date: 2016-02-03
天津达善生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the invention is to solve the problems of large waste water discharge, low product yield and poor product quality in the prior art, and to provide a method for separating and extracting branched-chain amino acids in fermentation broth by double-membrane continuous crystallization

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] a. Take 30L of L-leucine fermentation broth (L-leucine content 47.2g / L) and heat it at 80°C for more than 10min, add 0.05% alum and 0.5% diatomaceous earth, adjust the pH to 7.0, and enter the Membrane filtration system material tank (microfiltration membrane pore size 50nm), check whether the equipment is in normal condition, put cooling water into the condensation pipe, control the temperature at 40-50°C, start the microfiltration equipment, adjust the pressure difference between the inlet and outlet of the membrane to 0.3- 0.4MPa, using the different cut-off rates of microfiltration membranes for substances with different molecular weights, bacteria and proteins are trapped in the filter residue. When the pressure difference between the inlet and outlet membranes is significantly reduced, the microfiltration mother liquor is released. The recovery rate of supernatant L-leucine reaches 96.9%, the cell removal rate is greater than 99.9%, and the membrane flux reaches 62...

Embodiment 2

[0039] Get 30L of L-isoleucine fermentation broth (L-isoleucine content 45.5g / L) Repeat the operation according to the operating conditions of Example 1, change step f in Example 1 and add 7 times the weight of water, and then repeat the subsequent Operation steps, the obtained L-isoleucine weighs 1127.5g, the calculated yield is 82.6%, and the purity is 99.6%.

Embodiment 3

[0041] Get L-valine fermented liquid 30L (L-valine content 70.6g / L) Repeat operation according to the operation condition of embodiment 1, obtain L-valine and weigh 1707.1g, calculation yield is 80.6%, and purity is 99.5%.

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Abstract

The invention relates to a method for extracting branched chain amino acid from fermentation liquid by virtue of a double-membrane continuous crystallization method. According to the method, thalluses, most protein and pigments in the fermentation liquid are filtered in a cross flow manner by virtue of a double-membrane (micro-filtration membrane and nanofiltration membrane) separation system, then operation steps including sol deproteinization, activated carbon decoloration, vacuum concentration and crystallization, alkali dissolution, deproteinization decoloration, low-temperature iso-electric continuous crystallization and the like are carried out so as to obtain branched chain amino acid crystals with the purity not less than 98.5%, and the yield is not less than 80%; the thalluses are filtered by virtue of the micro-filtration membrane, and methods including centrifugation, filter press filter pressing and the like adopted in conventional fermentation liquid treatment are changed, so that the thalluses are thoroughly removed, and filtrate is clear and transparent; soluble protein in the fermentation liquid is further removed by virtue of sol, and a low-temperature iso-electric continuous crystallization manner is adopted, so that the product quality is improved; a traditional ion exchange process is omitted, so that the production of a lot of high ammonia-nitrogen wastewater is avoided, and the clean production of branched chain amino acid is realized.

Description

technical field [0001] The invention relates to a process for extracting branched-chain amino acids from fermentation liquid, and belongs to the technical field of amino acid production by fermentation. Background technique [0002] Branched-chain amino acids refer to neutral amino acids with branched aliphatic hydrocarbon chains on the α-carbon, including L-valine, L-leucine and L-isoleucine. The three branched-chain amino acids are all essential amino acids. Because of their special structures and functions, they play a particularly important role in life metabolism and have wide application and commercial value in the pharmaceutical and food industries. In terms of food, it is mainly used for food fortification to balance various amino acids and improve the nutritional value of food. In terms of medicine, branched chain amino acids can be used to produce compound amino acid infusions and a large number of drugs for the preparation of therapeutic special amino acids, such...

Claims

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Application Information

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IPC IPC(8): C07C229/08C07C227/40C07C227/42
Inventor 陈宁徐庆阳谢希贤张成林李燕军范晓光马跃超
Owner 天津达善生物科技有限公司
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