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Preparation method of cottonseed cake and meal feed containing lotus shell feeding compound enzyme

A feed compound enzyme and cottonseed cake technology, applied in animal feed, animal feed, application, etc., to achieve the effect of increasing protein content, low cost, and simple process

Inactive Publication Date: 2016-02-03
丁玉琴
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem mainly solved by the present invention: for the toxic substance free gossypol contained in the current cottonseed cake, it will

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0022] Weigh 200g of fresh lotus seed husks, put them in a pressure cooker and heat up to 150°C, process at 380kPa for 30min, pour them into a high-speed universal grinder for crushing, and then dry them in an oven at 105°C for later use; weigh 100g of wheat bran Put 20g of agar into a petri dish, raise the temperature to 70°C in a water bath, and after the agar melts, make up 900mL of distilled water to prepare a slant medium, inoculate 3g of Aspergillus niger into the slant medium, put it in an incubator and cultivate it at 30°C for 2 days; Rinse down the cultured slant spores with 300mL sterile water to obtain Aspergillus niger spore suspension, weigh 80g of the above dried lotus seed shells, 15g of wheat bran, 1g of ammonium sulfate, 0.5g of potassium dihydrogen phosphate and 150mL of distilled water Put it into a fermenter, add 200mL of Aspergillus niger spore suspension, and culture in a sealed manner at 35°C for 3 days; filter the fermented product to remove the filter r...

example 2

[0026]Weigh 250g of fresh lotus seed husks, put them in a pressure cooker and heat up to 155°C, process at 385kPa for 35min, pour them into a high-speed universal grinder for crushing, and then put them in an oven to dry at 108°C for later use; weigh 125g of wheat bran Put 25g of agar into a petri dish, heat up the water bath to 75°C, and after the agar melts, make up 950mL of distilled water to prepare a slant medium, inoculate 4g of Aspergillus niger into the slant medium, put it in an incubator at 35°C, and cultivate it for 2.5 days; Rinse down the cultured slant spores with 350mL sterile water to obtain a suspension of Aspergillus niger spores, weigh 85g of the above dried lotus seed shells, 18g of wheat bran, 1.5g of ammonium sulfate, 0.8g of potassium dihydrogen phosphate and 175mL of distilled water Put them together into a fermenter, add 250mL of Aspergillus niger spore suspension, and culture in a sealed container at 30°C for 4 days; filter the fermented product to rem...

example 3

[0030] Weigh 300g of fresh lotus seed husks, put them in a pressure cooker and raise the temperature to 160°C, process them under 390kPa high pressure for 40min, pour them into a high-speed universal grinder for crushing, and then put them in an oven to dry at 110°C for later use; weigh 150g of wheat bran Put 30g of agar into a petri dish, heat up the water bath to 80°C, and after the agar melts, make up 1000mL of distilled water to prepare a slant medium, inoculate 5g of Aspergillus niger into the slant medium, put it in an incubator at 40°C, and cultivate it for 3 days; Rinse down the cultured slant spores with 400mL sterile water to obtain Aspergillus niger spore suspension, weigh 90g of the above dried lotus seed shells, 20g of wheat bran, 2g of ammonium sulfate, 1g of potassium dihydrogen phosphate and 200mL of distilled water into the fermenter, add 300mL Aspergillus niger spore suspension, and culture at 45°C for 5 days; filter the fermented product to remove the filter ...

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Abstract

The present invention discloses a preparation method of cottonseed cake and meal feed containing lotus shell feeding compound enzyme and belongs to the field of feed. Aspergillus niger is added into a culture medium to be cultured to obtain an aspergillus niger spore suspension, lotus shells, wheat bran and other substances are added into the suspension to conduct sealed culture. An absolute ethyl alcohol is added into a filtrate to obtain a compound protease and the compound protease is separated and dried to obtain a lotus shell feeding compound enzyme. The cottonseed cake and meal is subjected to enzymolysis and detoxification and the enzymolyzed and detoxified cottonseed cake and meal, and the lotus shell feeding compound enzyme are subjected to mixed enzymolysis, granulation and drying to obtain the cottonseed cake and meal feed containing the lotus shell feeding compound enzyme. The aspergillus niger microorganism is used to conduct fermentation and culture. Free cotton phenol is combined with protein amino acids to form a combined cotton phenol, which not only achieves the purpose of detoxification, but also increases the protein content in the cottonseed cake and meal to prepare the lotus shell feeding compound enzymes to enable higher nutritional value of the feed.

Description

technical field [0001] The invention discloses a preparation method of cottonseed cake feed containing lotus seed husk feed compound enzyme, which belongs to the field of feed. Background technique [0002] Cottonseed cake is a by-product of cottonseed oil extraction. Weigh cake after oil extraction by pressing, and weigh meal after pre-press extraction or direct extraction. The by-product of extracting oil from cottonseed after shelling is called cottonseed cake. my country is a major cotton producing country in the world, with an annual output of more than 8 million tons of cotton. Among them, cottonseed accounts for about 50% to 55%, and the protein content of cottonseed is 35% to 38%. The crude protein content of cottonseed cake after oil extraction can reach 36% to 44%. However, because it contains free gossypol, which is toxic to livestock and poultry, the abundant protein resources have not been fully utilized. At present, the utilization rate of cottonseed meal f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K20/189A23K50/75
Inventor 丁玉琴王志慧林大伟
Owner 丁玉琴
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