A rapid detection device for microorganisms based on Mie scattering and spatial light modulator

A spatial light modulator and Mie scattering technology, applied in the field of medical biological microorganisms, cell tissue detection, can solve the problems of false positive test results, high cost, long time, etc., to achieve strong versatility, fast detection speed, and improved detection The effect of precision

Active Publication Date: 2019-03-29
NANJING MEDICAL UNIV
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  • Abstract
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Problems solved by technology

These methods are usually costly, requiring pre-cultivation of a certain number of microorganisms and adding specific culture reagents during the cultivation process, while the cultivation process takes a lot of time, and the possibility of false positive test results is high

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  • A rapid detection device for microorganisms based on Mie scattering and spatial light modulator
  • A rapid detection device for microorganisms based on Mie scattering and spatial light modulator

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Embodiment Construction

[0026] The present invention will be described in further detail below in conjunction with the accompanying drawings.

[0027] to combine figure 1 , the rapid detection equipment for microorganisms based on Mie scattering is mainly composed of a laser (1), an optical system (2), a detector spherical array frame (3), a sample pool (4), and an optical fiber (5). The diameter of the spherical array frame (3) of the detector is 203 mm, and the thickness of a single semi-arc shaft is 26 mm. The spherical array frame (3) of the detector is a spherical cage structure composed of four identical semi-arc shafts. The laser (1 ) laser wavelength is 632nm, the power is adjustable from 0mW to 100mW, the laser light emitted by the laser (1) is converged by the optical system (2), the focal point of the optical system (2) and the spherical center of the detector spherical array frame (3) Coincidentally, the back focal plane of the optical system (2) is 260mm, the sample cell (4) in the sha...

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Abstract

The invention discloses rapid microorganism detection equipment based on Mie scattering and a spatial light modulator. The equipment comprises a laser, an optical system, a detector spherical array frame, a cuvette and photoelectric detectors, wherein laser beam emitted by the laser forms parallel light after being collimated and is converged by the optical system and irradiated to a microorganism sample in the cuvette, a microorganism emits scattered light to the space after irradiated by the laser light, the scattered light is received by photoelectric detectors surrounding the sample pool, a computer performs analysis and calculation on received optoelectronic signals on the basis of the Mie scattering theory, and the form of the current microorganism can be detected in real time. Microorganisms are rapidly detected in real time by the device, the detection speed and accuracy are improved by means of the optical scattering principle, and signals are acquired, processed and analyzed in real time in the acquisition process.

Description

technical field [0001] The invention belongs to the field of detection of medical biological microorganisms and cell tissues, and relates to rapid detection equipment for microorganisms based on Mie scattering and a spatial light modulator. Background technique [0002] Microorganisms are all tiny organisms that are invisible or unclear to the naked eye. They are small and simple in structure, and usually can only be seen clearly with an optical microscope and an electron microscope. They are collectively referred to as microorganisms. Microorganisms include bacteria, viruses, molds, yeasts, etc. The detection of microorganisms is of great significance in food safety, disease diagnosis and control, and biological science research. The rapid detection of microorganisms requires the detection of target microorganisms in the shortest possible time with high sensitivity and specificity and simple operation as possible. Traditional microbial detection methods mainly include cul...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/47
Inventor 刘宾荣四海李智洋王炜何农跃吴小玲段磊朱松盛王伟朱禾
Owner NANJING MEDICAL UNIV
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