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A gene sequence and barcode technology, applied in the field of specific primer combination, can solve the problem of difficult to distinguish appearance, and shorten the identification time.
Active Publication Date: 2016-01-20
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES +1
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[0004] For multi-component agricultural products and traditional Chinese medicines, after processing, it is usually difficult to distinguish by appearance
Method used
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Embodiment 1
[0044] Utilize HHF1651 / HHR1822 primer combination, wherein
[0045] HHF1651 as SEQ ID NO.2 in the sequence listing;
[0046] HHR1822 as SEQ ID NO.3 in the sequence listing;
[0047] Perform PCR amplification identification.
[0048] 1. Experimental materials
[0049] 1.1 Plant material
[0050] The different safflower varieties tested are shown in Table 1.
[0051] Table 1 The name and origin of safflower varieties
[0052]
[0053] The other 17 different species of materials are: sunflower (Helianthusannuus), soybean (Glycinemax), corn (Zeamays), cotton (Gossypiumhirsutum), tomato (Solanumlycopersicum), wheat (Triticum), barley (Hordeumvulgare), cowpea (Vignaunguiculata) , Carrot (Daucus carota), Rapeseed (Brassicanapus), Capsicum (Capsicumannuum), Peanut (Arachishypogaea), Sesame (Sesamumindicum), Radish (Raphanus sativus), Rice (Oryza sativa), Tobacco (Nicotianatabacum), Arabidopsisthaliana.
[0054] 1.2 Enzymes and Reagents
[0055] Molecular biology reagents, TA...
Embodiment 2
[0083] Utilize HHF1651 / HHR1822 primer combination, wherein
[0084] HHF1651 as SEQ ID NO.2 in the sequence listing;
[0085] HHR1822 as SEQ ID NO.3 in the sequence listing;
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Abstract
The invention provides a safflower DNA bar code standard detection gene sequence and detection primer combination. The safflower DNA bar code standard detection gene sequence is characterized in that the bar code standard detection gene sequence is shown in the gene sequence SEQ ID NO.1. The gene is obtained after amplification through a polymerase chain reaction, the result is determined according to the detected sample amplification product, and if a band 171bp strip can be obtained after amplification in a specific manner, the situation that the detected sample contains the safflower component can be determined. The invention provides a method for specific detection of a safflower component. The method is helpful for identification of the safflower component and adulteration is prevented. When the method is compared with traditional morphological identification method, the obtained gene sequence facilitates to achieve safflower molecular identification and safflower identification time can be shortened effectively.
Description
technical field [0001] The invention relates to the field of species identification, in particular to a safflower species-specific gene sequence and a specific primer combination based on the gene sequence for specifically detecting safflower components. Background technique [0002] Safflower (scientific name: Carthamustinctorius, English name: Safflower) is an annual or biennial herb of the genus Safflower in the family Asteraceae. When it comes to safflower, it is easy for people to confuse safflower with saffron. Saffron (also known as saffron, saffron) is a plant of the genus Crocus (Crocus) in the family Iridaceae, and there is an essential difference between the two. Safflower is originally produced in Egypt, India, and the Mediterranean coast. It is wild and cultivated in the Soviet Union. It is widely cultivated in Korea and Japan. Now it is more produced in more than ten countries such as India, Canada, the United States, and Australia. Safflower is a foreign count...
Claims
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