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A Standard Gene Sequence of Guangxi Curcuma dna Barcode

A kind of Guangxi Curcuma curcuma and gene sequence technology, applied in the field of species identification, can solve the problem that there is no related gene sequence, and achieve the effect of shortening the identification time

Inactive Publication Date: 2015-07-29
GUILIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the DNA barcoding technology of the ITS gene can be used to carry out molecular identification of Zezhu in Guangxi. At present, there are only ITS gene sequences of other Zedoary species in Genbank, and there is no related gene sequence of ITS in Guangxi.

Method used

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  • A Standard Gene Sequence of Guangxi Curcuma dna Barcode
  • A Standard Gene Sequence of Guangxi Curcuma dna Barcode
  • A Standard Gene Sequence of Guangxi Curcuma dna Barcode

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0035] refer to figure 1 , a technical flow chart of the present invention.

[0036] 1. Collection and preservation of Ezhu specimens in Guangxi

[0037] Guangxi Curcuma from 10 different regions in Guangxi was collected, dried with silica gel, stored in silica gel, and the silica gel was replaced regularly.

[0038] 2. Extraction of Guangxi Curcuma DNA

[0039]Using CTAB method and alkaline lysis method to extract the DNA of Ezhu Guangxi, comparing the pros and cons of the two extraction methods, from the extraction results, the steps of the CTAB method are slightly cumbersome but the quality is better than the alkaline lysis method, so we use the CTAB method to extract ( Tiangen kit), and the extracted DNA samples were stored at -20°C for future use.

[0040] 3. Primer synthesis

[0041] The primers used in this example are as follows:

[0042] Forward primer: 5' TAG GTG AAC TGC GGA AGG AT 3';

[0043] Reverse primer: 5' GTG GTC TCG CCT GAT TGG 3';

[0044] Primers...

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Abstract

The invention discloses a curcama kwangsiensis DNA barcode standard gene sequence. The curcama kwangsiensis DNA barcode standard gene sequence is characterized in that the barcode standard detection gene is an ITS gene and has the gene sequence shown in the formula of SEQ ID NO.1. The sequencing result is subjected to manual proofreading, sequence splicing and Blast similarity searching in NCBI so that it is ensured that the obtained sequence is a required sequence. Curcama kwangsiensis species identification is realized based on morphology and molecular identification so that result reliability is guaranteed. The curcama kwangsiensis DNA barcode standard gene sequence is conducive to fast identification of curcama kwangsiensis, shortens identification time and improves identification accuracy.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a Guangxi zedoary DNA barcode standard gene sequence. Background technique [0002] Curcuma kwangsiensisS.G..Lee et C.F.Liang is a perennial herb in Zingiberaceae, widely distributed in Guangxi, Guangdong, Yunnan and Sichuan, and Curcuma phaeocaμlisval. Curcumawenyujin Y.H.Chen et al. C.Ling) is the 3 basic curcuma listed in the Chinese Pharmacopoeia. "Pharmacopoeia of the People's Republic of China" (2010 Edition) stipulates that the rhizomes of the above three plants are the commonly used traditional Chinese medicine "Curcuma", which has the effect of promoting qi, breaking blood, eliminating stagnation and relieving pain; Pain relieving, promoting qi circulation and relieving depression, clearing heart and cooling blood, promoting gallbladder and reducing jaundice. In addition, curcuma-like medicinal materials are often processed on the spot after collection. After proc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 王娟陈旭候艳芳曾建红付明磊刁珂白准黄凤香
Owner GUILIN MEDICAL UNIVERSITY
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