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A peanut DNA barcode standard detection gene and molecular identification method of peanut species

A standard detection and barcode technology, applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve problems such as consumer health hazards, peanut butter is susceptible to Aspergillus flavus, and shorten the identification time.

Active Publication Date: 2019-08-02
湖北元大粮油科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the other hand, peanut butter is mixed with sesame paste, and peanut butter is very susceptible to Aspergillus flavus, which will cause harm to the health of consumers

Method used

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  • A peanut DNA barcode standard detection gene and molecular identification method of peanut species
  • A peanut DNA barcode standard detection gene and molecular identification method of peanut species
  • A peanut DNA barcode standard detection gene and molecular identification method of peanut species

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] 1. Experimental materials

[0033] 1.1 Plant material

[0034] 16 different peanut varieties tested (7596, 7519, 7602, 7607, 7613, 21016, 21399, 21513, 21134, 21088, 21282, 21127, 21020, 21387, 21065, Zhonghua 5);

[0035] And other 16 different species of materials are: rapeseed, soybean, sesame, sunflower, oil palm, cotton, rice, corn, cowpea, pea, potato, radish, pepper, eggplant, Arabidopsis, tobacco.

[0036] 1.2 Enzymes and Reagents

[0037] Molecular biology reagents, TAKARA Taq DNA polymerase, 10×PCR buffer, dNTP Mixture (10mM), MgCl 2 purchased from Dalian Bao Biological Company. PCR primers were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0038] 1.3 Experimental Instruments

[0039] PCR instrument: DNA Engine Dyad Peltier Thermal Cycler (Bio-Rad, USA);

[0040] Nucleic acid electrophoresis instrument: DYYIII type YY0115-94 (Beijing Liuyi Instrument Factory);

[0041] Gel imaging system: Gel Doc XR System (Bio-Rad, USA); ...

Embodiment 2

[0062] 1. Experimental materials

[0063] 1.1 Plant material

[0064] Peanut butter (Sijibao brand), mixed sesame paste (Liubiju brand), sesame paste (commercially available), peanut candy (Big White Rabbit brand), fried sesame seeds (commercially available).

[0065] 1.2 Enzymes and Reagents

[0066] Molecular biology reagents, TAKARA Taq DNA polymerase, 10×PCR buffer, dNTP Mixture (10mM), MgCl 2 purchased from Dalian Bao Biological Company. PCR primers were synthesized by Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0067] 1.3 Experimental Instruments

[0068] PCR instrument: DNA Engine Dyad Peltier Thermal Cycler (Bio-Rad, USA);

[0069] Nucleic acid electrophoresis instrument: DYYIII type YY0115-94 (Beijing Liuyi Instrument Factory);

[0070] Gel imaging system: Gel Doc XR System (Bio-Rad, USA);

[0071] Other instruments include: constant temperature water bath, electronic balance, centrifuge, vortex instrument, pure water instrument, constant temp...

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PUM

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Abstract

The invention provides a peanut DNA bar code standard assay gene sequence and an assay primer combination. The bar code standard assay gene sequence is the following gene sequence SEQ ID No.1 of a gene. The gene is amplified through a polymerase chain reaction, the result is judged according to the size of an assayed sample amplified product, and if a 165bp stripe can be specifically amplified, it can be judged that an assayed sample contains a peanut ingredient. The invention further provides a method for specifically detecting the peanut ingredient. The peanut ingredient can be identified, adulteration and impurity use are avoided, and health of customers is guaranteed. Compared with a traditional morphological identification method, the obtained gene sequence can achieve molecular identification of peanuts, and the identification time of the peanuts can be effectively shortened.

Description

technical field [0001] The invention relates to the field of species identification, in particular to a peanut species-specific gene sequence and a specific primer combination based on the gene sequence for specifically detecting peanut components. Background technique [0002] Peanut (scientific name: Arachis hypogaea, English name: peanut), formerly known as groundnut, is an annual herb of the family Rosaceae and leguminous. Peanut (Arachis hypogaea) is the only cultivated species of the genus Arachis. Peanut is an important source of edible protein and high-quality edible oil, and it is also one of the most important oil crops in China. Peanut kernels have high oil content and rich nutrition (45-55% oil; 27-30% protein; 6-23% carbohydrates; 2% cellulose), the protein content of oil meal reaches 50%, and it is also rich in vitamin E and B. is good fodder. Peanut kernels can also be further made into peanut milk powder, yogurt, pastry and puffed food, etc. Peanut stems ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895
Inventor 张丽覃瑞刘虹李刚祝贺邱秀双王龙体
Owner 湖北元大粮油科技有限公司
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