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Microbe capable of producing aflatoxin B1 (AFB1) degrading enzyme and application thereof

A kind of aflatoxin, degrading enzyme technology, applied in the field of microorganisms, can solve problems such as aflatoxin pollution

Active Publication Date: 2016-01-20
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a strain producing aflatoxin detoxification enzyme and its application, effectively solving the problem of aflatoxin pollution

Method used

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  • Microbe capable of producing aflatoxin B1 (AFB1) degrading enzyme and application thereof
  • Microbe capable of producing aflatoxin B1 (AFB1) degrading enzyme and application thereof
  • Microbe capable of producing aflatoxin B1 (AFB1) degrading enzyme and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1: the screening of strain

[0045] (1) Screening samples

[0046] Rotten Peanut Shells

[0047] (2) Medium

[0048] Every 1L of the enriched medium contains: coumarin 1g, (NH 4 ) 2 SO 4 5g, NaCl8.5g, the pH of the enrichment medium is natural;

[0049] The primary screening medium per 1L contains: coumarin 1g, (NH 4 ) 2 SO 4 5g, KH 2 PO 4 2.5g, MgSO 4 1g,NaH 2 PO 4 ﹒ 12H 2 O0.5g, FeSO 4 ﹒ 7H 2 O0.1g, CaCl 2 0.1g, 20g of agar, the pH of the primary screening medium is 6.0-6.5;

[0050] Each 1 L of the slant medium contains: 200 g of potatoes, 20 g of glucose, and 20 g of agar, and the pH of the slant medium is natural;

[0051] Each 1L of the seed medium contains: 200g of potatoes, 20g of glucose, 1.0mL of Tween80, and the pH of the seed medium is natural;

[0052] Each 1L of the re-screened medium contains: 30g of sucrose, NaNO 3 2.0g, K 2 HPO 4 ·3H 2 O1.0g, KCl0.5g, MgSO 4 0.5g, FeSO 4 ·7H 2 O0.01g, pH natural;

[0053] (3) Th...

example 2

[0068] Example 2: The culture condition optimization of CCTCCNO:M2015181

[0069] (1) Strain: CCTCCNO:M2015181

[0070] (2) Method steps

[0071] The fermentation medium is the initial medium (g / L): 30 sucrose, NaNO32.0, K2HPO4·3H2O1.0, KCl0.5, MgSO40.5, FeSO4·7H2O0.01. The initial fermentation conditions are: natural pH, inoculum size 10%, liquid volume 50ml / 250ml, 28°C, shaker speed 180r / min, fermentation cycle 48h.

[0072] The effects of fermentation temperature, initial pH, fermentation cycle, inoculum amount, and liquid volume on the degradation rate of AFB1 of CCTCCNO:M2015181 were investigated by single factor experiment.

[0073] a. optimal test of fermentation temperature

[0074] according to figure 1 It can be seen that the degradation rate of AFB1 does not change much under the condition of 25-34°C, but it has a greater impact on the biomass, and microorganisms cannot grow under the condition of 37°C and 40°C. In the case of not seriously affecting the gro...

example 3

[0083] Example 3: CCTCCNO: M2015181 medium optimization

[0084] a. Carbon source optimization

[0085] CCTCCNO: M2015181 fermentation medium carbon source optimization results are as follows Figure 7 shown. It can be seen that the AFB1 degradation rate corresponding to most of the carbon sources is very considerable, reaching more than 80%, and the AFB1 degradation rate corresponding to molasses, corncob powder, and corn flour is close to 100%, but the corncob powder group has a certain degree of degradation in the extraction stage. The emulsification phenomenon, for the sake of the rigor of the experiment, this set of data is not considered for the time being. The lowest degradation rate was 50% in the orange peel powder group.

[0086] CCTCCNO:M2015181 can grow well in the fermentation medium of molasses, sodium carboxymethyl cellulose, corn cob powder, and orange peel powder group, which shows that CCTCCNO:M2015181 can utilize these industrial and agricultural by-produ...

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PUM

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Abstract

The invention discloses a microbe capable of producing an AFB1 degrading enzyme, i.e., Cladosporium uredinicola HSK8. The microbe is preserved in China Center for Type Culture Collection on March 31, 2015, with an accession number of CCTCC No. M 2015181. The microbe can produce the AFB1 degrading enzyme through fermentation of industrial and agricultural by-products or waste consisting of molasses, corncob powder and orange peel powder, so cost is substantially saved.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and the content is a bacterial strain capable of effectively degrading aflatoxin B1 (AFB1) Cladosporium uredosporum ( Cladosporium uredinicola HSK8) and its application. technical background [0002] Aflatoxin B1 was discovered in 1960. At that time, in the UK, a large number of turkeys died suddenly because they were fed peanut meal containing aflatoxin. Since then, more than 20 aflatoxins have been isolated and 18 have been identified, the most common of which are B 1 , B 2 , G 1 , G 2 , M 1 and M 2 . Class B aflatoxins are named for their ability to emit blue fluorescence under ultraviolet light. Similarly, class G aflatoxins emit green fluorescence under ultraviolet light. Aflatoxins M1, M2 It is so named because it was first found in milk. Among the aflatoxins, aflatoxin B1 (AFB1) is the most toxic. AFB1 causes a large amount of food pollution every year and is harmful to h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N9/00C12R1/645
Inventor 蔡俊邵帅王常高杜馨林建国
Owner HUBEI UNIV OF TECH
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